鸡传染性贫血病疫苗免疫母鸡后子代雏鸡外周血液T细胞数量及免疫球蛋白含量变化  

Dynymic Changes of the Number of T Cell and the Content of Immunoglobulina in the Peripheral Blood of Chicks From Hens Vaccin AtedWith Chicken Infectious Anemia (CIA)

作　　者：葛铭 张瑞莉[1] 郑世民[1] 李德军[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《黑龙江畜牧兽医》2001年第12期1-2,共2页Heilongjiang Animal Science And veterinary Medicine

摘　　要：应用免疫SPA菌体花环法和间接ELISE法对鸡传染性贫血病 (CIA)疫苗免疫母鸡后 ,其子代雏鸡外周血液T细胞数量和血清IgG ,IgM ,IgA含量进行了动态研究。结果发现 :CIA疫苗免疫母鸡后 ,其子代雏鸡外周血液T细胞数量和IgG、IgM、IgA含量均不同程度地高于未免疫的相应对照雏鸡 ,表明CIA疫苗免疫母鸡后 ,其子代雏鸡全身的体液免疫和细胞免疫功能明显增强。而CIAV强毒攻击子代雏鸡后 ,未免疫的子代雏鸡外周血液上述各项指标均明显低于疫苗免疫的子代雏鸡 ,这与CIAV强毒感染雏鸡后 ,其免疫器官组织 ,尤其是胸腺严重损害 ,淋巴细胞变性坏死等密切相关 。This experiment was undertaken dynamic study of the number of T cells and the content of the immunoglobulin(Ig)G,IgM,IgAin the peripheral blood of chicks from hens vaccinated with chicken infectious anemia(CIA) with staphylococcal protein A(SPA) rosette test and indirect enzyme linked immunosorbent assay(ELISE).The resules were as follows:after vaccinated the hens with CIA,the number of T cells and the content of IgG,IgM,IgA in the peripheral blood of chicks from hens vaccinated different degree increased in comparison with those of non-vaccinated controls.These results showed that the function of humoral immune and T cellular immune of chicks from hens vaccinated with CIA vaccine could be markedly enhanced.Post challenged of filial generation chicks from hens with chicken infectious anemia virus(CIAV),the above mentioned very item in the periperal blood of chicks from hens non-vaccinated with CIA vaccine were lower than those of vaccinated-challenged chicks.This matter has immune organs and tissues of chicks infected with CIAV, especial thymus being injured seriously, to do with lymphocytes degeneration and necrosis.This maybe was basis of immune function low of chicks infected with CIAV.

关 键 词：母鸡 传染性贫血病疫苗 免疫 子代雏鸡 外周中血液T细胞 细胞数量 免疫蛋白含量 

分 类 号：S858.312.5[农业科学—临床兽医学]