1日龄雏鸡接种超抗原SEB和MDV后脾与胸腺淋巴细胞IFN-γ的体外诱生  

The IFN-γ motivation in vitro of the spleen and thymus after the inoculation of SAG-SEB and MDV at one day old chicken

作　　者：魏萍 刘宝全[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《黑龙江畜牧兽医》2001年第12期5-6,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江自然科学基金项目 (2 5 0 0 5 7)

摘　　要：为研究超抗原 (SAg)在禽类诱导的免疫机理和进一步揭示MDV(马立克氏病病毒 )感染的免疫学规律 ,采用NO-释放法 ,设计用雏鸡骨髓巨噬细胞为靶细胞检测 1日龄雏鸡接种超抗原SEB和MDV后脾与胸腺淋巴细胞IFN -γ体外诱生的动态变化。结果发现 ,超抗原SEB(SAG -SEB)可提高脾与胸腺淋巴细胞IFN -γ体外诱生水平 ,其中 ,高剂量(1ng/只 )引起短时加强后下降 ,而低剂量 (0 .0 1ng)则出现缓升缓降现象 ;而MDV接种则抑制脾与胸腺淋巴细胞IFN-γ体外诱生 ;SAg -SEB能增强雏鸡细胞免疫 ,主要表现于接种初期 (1- 10dPI) ,而MDV却在接种后 1- 2 5d间降低细胞免疫 ;说明超抗原SEB和MDV虽然均可引起鸡T细胞增殖 ,但对鸡免疫细胞IFN -γ的体外诱生作用不同。In order to study the immunity of SAg and MDV in chicken,a nitric oxide production bioassay was used to measure the IFN-γ production of chicken Spleen and Thymus lymphocyte in vitro.The results showed that the IFN-γ production of Spleen and Thymus lymphocyte in vitro was enhanced by SAg-SEB , respectively,and by higher dose SAg-SEB the IFN-γproduction was enhanced in short time then decreased,and by lower dose SAg-SEB the IFN-γproduction was enhanced and decreased slowly.But the IFN-γproduction of Spleen and Thymus lymphocyte in vitro was inhibited from MDV infection .The action of SAg-SEB appeared in 1-10 days PI and MDV in 1-25 days; All of these demonstrated that both of SAg-SEB and MDV induced T cell proliferating ,but have showed different way in IFN-γproduction.

关 键 词：超抗原SEB MDV 脾 胸腺淋巴细胞 鸡IFN-γ 体外诱生 1日龄雏鸡 接种 

分 类 号：S858.312.4[农业科学—临床兽医学]