去势对大鼠阴道性功能的影响  

The Effect of Castration on Sexual Function of Rat Vagina

作　　者：张彩霞[1] 胡礼泉[1] 代赵明[2] 

机构地区：[1]武汉大学医学院泌尿外科男科学研究中心,武汉430071 [2]武汉大学医学院生化教研室,武汉430071

出　　处：《医学新知》2002年第1期31-32,共2页New Medicine

摘　　要：目的　探讨雌激素在维持女性性反应中的分子学机制。方法　 40只雌性成年SD大鼠随机分为 3组 :去势组 (10只 )、替代组 (10只 )及对照组 (2 0只 )。通过阴道涂片筛选出待取材的动物 ,于术后第 14d取出阴道组织 ,用分光光度计测定其一氧化氮合酶 (NOS)活性。结果　去势组NOS活性显著低于对照组 (P <0 .0 1) ,替代组NOS活性显著上升 (P <0 .0 1) ,并接近对照组正常水平 (P >0 .0 5 )。结论　雌激素可通过调节阴道NOS活性来维持女性性反应。Objective To investigate the effect of castration on sexual function of rat vagina and explore the molecular mechanism of estrogen in maintaining the female sexual response.Methods Forty mature female SD rats were randomly divided into three groups:castration group (oophorectomy,n=10);estrogen replacement after oophorectomy (n=10);and sham-operated control (n=20).The needed sampling rats were selected by wet mount smears of vaginal secretions.On the 14th day after operation,vagina tissues were harvest.NOS activity was detected with spectrophotometry.Results NOS activity decreased significantly in vaginal tissues of the oophorectomy group compared with those of control group (P<0.01),while estrogen replacement could significantly increase the NOS activity (P<0.01) to the level of control(P>0.05).Conclusion Estrogen may maintain the female sexual response by regulating NOS activity

关 键 词：女性性功能障碍 雌激素 一氧化氮合酶 阴道 大鼠 

分 类 号：R711.7[医药卫生—妇产科学]