酶/酶免疫电极最佳制备方式的确定及其微观分析  

The Determination of the Best Modified Way and Microscopical Analysis for Enzyme/immunoelectrodes

作　　者：董飒英 王洪仁 高玉柱 陈光章[1] 吕鸣祥[2] 田波义[2] 

机构地区：[1]七二五研究所青岛分部,山东青岛266071 [2]天津大学化工学院,天津300072

出　　处：《电化学》2002年第1期66-72,共7页Journal of Electrochemistry

基　　金：国防预研基金 (编号 :JW 96 0 4 0 1)资助项目

摘　　要：利用电化学方法测定各种实验条件下酶电极、酶免疫电极的电化学响应特性 ,以期确定这种新型生物电极的最佳制备条件 。In this paper,the electrochemical response characteristics of enzyme and immuno electrodes were studied by cyclic voltammetry under different conditions. Our goals were to determine the best modified way of bioelectrode,which was the base of biosensor's application. 30U GOD and 0.8 mg/mL SEC1 antibody were suitable to bioelectrode preparation and detection. The microscopical analysis was finished with scan microscopy.

关 键 词：酶电极 酶免疫电极 响应特性 免疫反应 最佳制备条件 电化学检测 肠毒素 临床检测 

分 类 号：O657.1[理学—分析化学]