DHP的^(13)C同位素示踪及固体^(13)C NMR分析  

CARBON 13-ENRICHMENT OF DEHYDROGENATION POLYMERS OF MONOLIGNOLS TRACED BY SOLID-STATE ^(13) C NUCLEAR MAGNETIC RESONANCE

作　　者：顾瑞军[1] 谢益民[1] 伍红[1] 赖燕明[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640

出　　处：《林产化学与工业》2002年第1期1-6,共6页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目 (2 9974 0 11;30 170 75 6 ) ;霍英东教育基金资助项目 (710 6 6 ) ;广东省教育厅千百十工程优秀人才基金资助项目

摘　　要：以侧链α位带13 C同位素标记的松柏醇葡萄糖苷作为起始物 ,在实验室中模拟木质素生物合成过程人工合成脱氢聚合物 (DHP) ,得到带13 C标记的DHP。用高分辨率固体13 CNMR和红外光谱对DHP进行了分析。红外分析结果显示 :该实验条件下用松柏醇葡萄糖苷合成的DHP同天然银杏木材的磨木木质素 (MWL)在组成和结构上有较好的相似性。13 CNMR证明了 β O 4、β β、β 5和松柏醇结构为DHP的主要结构 ,另有少量香兰素结构和αGuaiacyl type dehydrogenation polymers(DHPs)were prepared from coniferin and coniferin (α 13 C)by peroxidase,glucose oxidase and β glucosidase.The DHPs were analyzed by high resolution solid state carbon 13 nuclear magnetic resonance ( 13 C NMR) spectroscopy and infrared spectroscopy.The result of IR indicated that the chemical structure of DHP was in well agreement with MWL prepared from ginkgo wood.It was proved that β O 4,β β,β 5 and coniferyl alcohol structures were the main substructures in DHPs although there were some minor substructures such as vanillin and α methylene units according to the determination of CP/MAS 13 C NMR spectra.

关 键 词：脱氢聚合物 木质素 碳-13 同位素示踪 松伯醇 生物合成 同位素标记 核磁共振 

分 类 号：O636.2[理学—高分子化学]