菌丝体的膜流动性对去饱和酶活性的影响  

Effects of Mycelial Bio-membrane Fluidity on the Activity of Desaturases

作　　者：康亦兼[1] 咸漠[2] 王君霞[2] 程铁欣[2] 李文兴[2] 毕颖丽[2] 

机构地区：[1]青岛化工学院化工系,山东青岛266042 [2]吉林大学化学系,吉林长春130023

出　　处：《分子催化》2002年第1期1-4,共4页Journal of Molecular Catalysis(China)

基　　金：国家自然科学基金资助项目 (批准号 :2 98730 18)

摘　　要：以 DPH和 ANS标记深黄被孢霉的生物膜 ,考察了生物膜流动性的变化与去饱和酶活性的关系 .结果表明 ,生物膜流动性下降 ,活化了Δ 12和Δ6去饱和酶 ,进而合成了大量的亚油酸和亚麻酸 ;生物膜脂中脂肪酸不饱和度的提高 ,可致使生物膜的流动性提高 .这可能是在低温下 ,微生物保持正常的物质和能量代谢的自我调控 。Effects of mycelial bio\|membrane fluidity on the activity of desaturases from Mortierella isabellina were investigated by fluorescence spectrophotometry with ANS and DPH as probes. The results show that the contents of linoleic and linolenic acids are increased due to the decrease of mycelial bio\|mambrane fluidity activation of Δ12 and Δ6 desaturases. The increase of the contents of these two acids causes the increase of the unsaturation degree of fatty acids in mycelial bio\|membrane and the increase of the fluidity of bio\|membrane. This change could be the reason for microorganisms to keep their seef\|controlling mechanism at low temperature.

关 键 词：流动性 去饱和酶 高不饱和脂肪酸 生物膜 菌丝体 酶活性 生物合成 调控 

分 类 号：Q936[生物学—微生物学]