9.1C3对CD2和CD3诱导的杀伤作用的影响及其机制探讨  

Study on the effects and its mechanism of 9.1C3 on CD2 or CD3 mediated cytotoxicities

作　　者：欧阳为明[1] 李琦[1] 刘雪松[1] 张云[1] 贾卫[1] 李德敏[1] 夏海滨[1] 金伯泉[1] 

机构地区：[1]第四军医大学基础部免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2002年第2期169-171,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目;No. 39770381

摘　　要：目的　　探讨９．１Ｃ３分子对ＣＤ２和ＣＤ３诱导的ＰＢＭＣ杀伤作用的影响及其作用机制。方法以活化ＰＢＭＣ为效应细胞，采用重导向杀伤实验（ｒｅｄｉｒｅｃｔｅｄ　ｃｙｔｏｔｏｘｉｃｉｔｙ　ａｓｓａｙ，ＲＣＡ），观察９．１Ｃ３对ＣＤ２、ＣＤ３介导效应细胞杀伤Ｐ８１５细胞作用的影响。以Ｊｕｒｋａｔ细胞为模型，用相应的抗体刺激细胞，通过荧光分光光度计，测定９．１Ｃ３对ＣＤ２、ＣＤ３诱导［Ｃａ２＋］ｉ升高的影响。结果９．１Ｃ３　ｍＡｂ能显著抑制ＣＤ２　ｍＡｂ介导活化ＰＢＭＣ对Ｐ８１５细胞的杀伤作用，但对ＣＤ３　ｍＡｂ介导杀伤的抑制作用较弱。以Ｊｕｒｋａｔ细胞为模型，发现ＣＤ２　ｍＡｂ经羊抗鼠Ｉｇ（ｇｏａｔ　ａｎｔｉ　ｍｏｕｓｅ　Ｉｇ，　ＧＡＭ　Ｉｇ）交联后能诱导［Ｃａ２＋］ｉ的升高，当同时加入９．１Ｃ３　ｍＡｂ时，［Ｃａ２＋］ｉ的升高受到抑制；ＣＤ３　ｍＡｂ在没有ＧＡＭ　Ｉｇ交联时即能引起［Ｃａ２＋］ｉ的升高，而９．１Ｃ３　ｍＡｂ对ＣＤ３　ｍＡｂ诱导［Ｃａ２＋］ｉ的升高有赖于ＧＡＭ　Ｉｇ的交联。结论９．１Ｃ３分子对ＣＤ２和ＣＤ３介导的杀伤的抑制程度不同。抑制杀伤细胞脱颗粒依赖的［Ｃａ２＋］ｉ升高可能是９．１Ｃ３分子抑制活?Aim To explore the effects and its mechanism of 9.1C3 on CD2 or CD3 mediated cytotoxicities of activated PBMC. Methods The effects of 9.1C3 on CD2 or CD3 induced cytotoxicities were investigated by using redirected cytotoxicity assay, in which activated PBMC were used as effectors, and Fc receptor expressing P815 were used as target cells. The effects of 9.1C3 on CD2 or CD3 mediated [Ca2+]i increase in Jurkat cells were detected by spectrofluorometer. Results CD2 mediated cytotoxicity was inhibited significantly by 9.1C3 mAb, whereas CD3 mediated cytotoxicity was inhibited by 9.1C3 mAb to less extent. CD2 mediates [Ca2+]i increase in crosslinking dependent manner, and [Ca2+]i increase was inhibited in the presence of 9.1C3 mAb. However, CD3 mediates [Ca2+]i increase in crosslinking independent manner, and the increase was only inhibited in the presence of 9.1C3 mAb and crosslinker. Conclusion 9.1C3 can inhibit CD2 or CD3 molecule mediated cytotoxicities to different extent. The inhibition of CD2 mediated [Ca2+]i increase may be one mechanism of inhibitory effect of 9.1C3 on CD2 molecule mediated cytotoxicity. The lower inhibitory effect of 9.1C3 on CD3 molecule mediated cytotoxicity may be due to the absence of crosslinker in the experiment of incubating effect cells with antibodies in RCA.

关 键 词：活化PBMC 9.1C3 抑制型杀伤细胞受体 单克隆抗体 CD2 CD3 

分 类 号：R392.11[医药卫生—免疫学]