抗BrdU单克隆抗体在凋亡细胞检测中的应用研究  

Application of anti-BrdU mAb to detecting apoptotic cells

作　　者：徐志伟[1] 吴国庆[1] 李少华[1] 苗乃法[1] 魏兵[1] 冯永堂[1] 邓鸿业[2,3,4,5] 

机构地区：[1]山东省潍坊医学院免疫学研究所,山东潍坊261042 [2]北京大学医学部免疫学系 [3]mAb [4]细胞凋亡 [5]检测

出　　处：《细胞与分子免疫学杂志》2002年第2期156-157,160,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨将自制的抗ＢｒｄＵ　单克隆抗体（ｍＡｂ）用于ＴＵＮＥＬ法检测凋亡细胞的可能性。方法分别用ＴＵＮＥＬ免疫荧光法和免疫组化技术，检测地塞米松诱导的凋亡胸腺细胞和射线照射的大鼠皮肤组织中的凋亡细胞。结果用ＴＵＮＥＬ免疫荧光法和ＴＵＮＥＬ免疫组化法法检测发现，凋亡细胞的细胞核分别呈明显的荧光或酶底物着色，而非凋亡细胞不显示荧光或无酶底物着色。结论该抗ＢｒｄＵ　ｍＡｂ用于ＴＵＮＥＬ法，能够检测单个细胞标本或组织切片中的凋亡细胞，为国内开展凋亡细胞的检测，提供了一种新的方法。Aim To explore the possibility of the application of self-made anti-BrdU mAb in TUNEL assay for detecting apoptotic cells. Methods TUNEL-based immunoflourescent and immunohistochemical assays were used respectively to examine apoptotic cells in Dex-induced thymocytes and γ-ray irradiated rat skin tissue. Results It was found that the nuclear of apoptotic cells exhibited bright flourescence or brown color,while non-apoptotic cells did not. Conclusion This anti-BrdU mAb can be used to detect apoptotic cells in single cell samples and in tissue section with immunoflourescence or immunohistochemical technique, thus providing a new mefhcd for detection of apoptotic cells at home.

关 键 词：溴化脱氧尿嘧啶 MAB 细胞凋亡 检测 单克隆抗体 

分 类 号：R392.33[医药卫生—免疫学]