关于NaNO_2-Al(NO_3)_3-NaOH比色法测定总黄酮方法的探讨  

Discussion about NaNO_2 - Al (NO_3)_3 - NaOH Colorimetry for Determination of Total Flavonoids

作　　者：郭亚健[1] 范莉[1] 王晓强[1] 张兰珍[1] 

机构地区：[1]北京中医药大学中药学院中药化学教研室,北京100029

出　　处：《药物分析杂志》2002年第2期97-99,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:探讨NaNO_2-Al(NO_3)_3-NaOH定量方法的专属性。方法:选用多种黄酮及非黄酮化合物,与NaNO_2、Al(NO_3)_3、NaOH试剂反应后测定400～700nm吸收曲线。结果:有些黄酮类物质在500nm处无最大吸收或吸收很弱,而有些非黄酮类物质在500nm处有最大吸收或有较强吸收。结论:以芦丁为对照品,NaNO_2-Al(NO_3)_3-NaOH比色法测定总黄酮方法专属性差。Objective: To probe into the speciality of NaNO2 - Al ( NO3) 3 - NaOH colorimetry for determination of total flavonoids. Method: Make a series of flavonoids and non - flavonoids react with NaNO2, Al (NO3)3, NaOH solution, then mensurate their absorption curves at the range of 400-700 nm. Result: At the wavelength 500 nm, some flavonoids showed no λmax or very weak absorption, and to the opposite, some non -flavonoids have maximized absorption or have upper absorption. Conclusion: It is not exclusive to determine total flavonoids by u-sing NaNO2 - Al ( NO3) 3 - NaOH colorimetry with rutin as reference substance.

关 键 词：比色法 总黄酮 含量测定 NaNO2-Al(NO3)3-NaOH 

分 类 号：R284[医药卫生—中药学]