反相高效液相色谱荧光检测法测定人血浆中阿呋唑嗪浓度  

Determination of Alfuzosin in Human Plasma by RP - HPLC with Fluorometric Detection

作　　者：姚宏伟[1] 金涌[1] 李俊[1] 张运芳[1] 丁秀年 徐叔云[1] 

机构地区：[1]安徽医科大学临床药理研究所,合肥230032

出　　处：《药物分析杂志》2002年第2期127-129,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立血浆中阿呋唑嗪的反相高效液相荧光检测方法。方法:采用Shimadzu LC-6高效液相色谱仪,ShimadzuC_(18)柱(150mm×4.6 mm),0.02 mol·L^(-1)磷酸盐缓冲液(pH2.5)-乙腈(40:60)为流动相,激发波长374nm,发射波长378nm,流速:1mL·min^(-1)。结果:血浆中阿呋唑嗪能得到较好的分离,无明显的干扰峰。本方法专属性好,可信度高。最低检测浓度为0.78ng·mL^(-1),最低检测限是39pg,线性范围0.78～50 ng·mL^(-1),标准曲线:Y=8648.7X+767.8(r=0.999,P<0.05),回收率高于70%,日间、日内RSD均在10％以下。结论:符合生物样品的分析要求。Objective: To establish a RP - HPLC method for determination of alfuzosin in human plasma. Methods: The chromatographic column was a Shimadzu C18 column (150 mm ×4. 6 mm). The mobile phase, 0.02 mol·L-1 phosphate buffer (pH 2. 5) - acetonitrile (40:60) , was used at a flow rate of 1.0 mL·min-1. The fluorimetric excitation and emission wavelengths were set at 374 nm and 378 nm, respectively. The serum samples were alkalized with NaOH and extracted with diethyl ether. The organic phase was evaporated to dryness with N2 stream, and the residues were dissolved with 0. 02 mol·L-1phosphate buffer ( pH 2. 5) - acetonitrile (90: 10). Results: Under the condition of test, alfuzosin was well separated and there had not obvious interactive peak in plasma. The results of detection could represent concentration of primary drug. The lowest concentration of detection was 0. 78 ng·mL-1, the lowest limit of detection was 39 pg. The linear ranges was 0. 78 -50 ng ·mL-1, the linear regression equation was Y = 8 648. 7X + 767. 8 ( r = 0. 999, P < 0. 05 ). Recovery ratio of extraction was higher than 70% , RSD of intra - day and inter - day were lower than 10%. Conclusion: All of those satisfied analytical requirement of biological preparation.

关 键 词：反相高效液相色谱法 阿呋唑嗪 血浆 荧光检测 测定 血药浓度 药代动力学 

分 类 号：R969.1[医药卫生—药理学]