L20B细胞吸附法用于环境水中病毒的分离培养  

TO ISOLATE POLIO VIRUSES IN ENVIRONMENTAL WATER WITH THE METHOD OF CELL ABSORPTION

作　　者：邵荣标 吴巨飞 刘秀兰 丁昌慧 严建国 汤权 祁新辉 

机构地区：[1]江苏省盐城市卫生防疫站,盐城224002

出　　处：《南通医学院学报》2002年第1期27-28,共2页ACTA Academiae Medicinae Nantong

摘　　要：目的 :建立一种用于检测外环境水中脊髓灰质炎病毒的方法。方法 :细胞吸附浓集法 :将自然沉清的水样抗菌、等渗处理后 ,加入适量的 L 2 0 B细胞 ,混匀 ,充分吸收水中的脊髓灰质炎病毒 ,然后收集细胞培养、分离病毒。同时用 Al Cl3- Na Cl沉淀法浓集病毒 ,接种 L2 0 B细胞分离病毒 ,作为对照。结果 :4 0份水样 ,用 L2 0 B细胞吸附法检出10株脊髓灰质炎病毒 ,用 Al Cl3- Na Cl沉淀法未检出脊髓灰质炎病毒。结论 :新建立的细胞吸附浓集法可用于外环境水中的脊髓灰质炎病毒的检测 ,且检测的敏感性明显高于 Al Cl3- Na Cl沉淀法。Objective:To set up a simple,practical method for detecting the polio viruses in the environmental water.Methods:Adding L20B cells into the large volume water,which was sterilized with germicide and was balanced with balancing salts.Collecting the cells after they fully reacted with polio viruses,then culturing viruses,with the method of AlCl 3-NaCl sedimentation as control.Results:Of 40 water samples,the polio viruses were detected in 10 samples with L20B cell absorption method,and no polio viruses were detected in all samples with the sedimentation method.Conclusions:The L20B cell absorption method might be used for detecting the polio viruses in environmental water,and it was more sensitive than the method of AlCl 3-NaCl sedimentation.

关 键 词：脊髓灰质炎病毒 水 L20B细胞 吸附 环境水 

分 类 号：R373.22[医药卫生—病原生物学] R123.1[医药卫生—基础医学]