肝细胞膜钠泵活性变化在胆红素钙结石成石过程中的作用  

Effects of Activities of Na^+-K^+-ATPase in Plasma Membranes of Hepatocytes in the Formation of Calcium Bilirubinate Gallstone

作　　者：庄文[1] 肖路加[1] 左凤琼[2] 东云华[3] 吴忠舜[4] 吴兆锋[2] 

机构地区：[1]四川大学华西医院普外科,成都610041 [2]四川大学华西基础医学与法医学院生化教研室 [3]四川大学华西基础医学与法医学院检测中心 [4]四川大学华西公共卫生学院病理科

出　　处：《中国普外基础与临床杂志》2002年第2期73-75,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　探讨肝细胞膜钠泵 (Na+ K+ ATPase)活性变化在胆红素钙结石成石过程中的作用。方法　采用胆红素钙结石兔模型进行研究 ,对照组 2 8只 ,胆道不全梗阻组 (BO组 ) 3 6只 ,胆道不全梗阻加感染组 (BOI组 ) 3 9只 ,各组再按术后处死时间 3d、7d、14d及 2 0d时相分组。动态测定各组各时相肝细胞膜钠泵活性、肝细胞钙及胆汁中胆汁酸含量。结果　BO组及BOI组肝细胞膜钠泵活性及胆汁中胆汁酸含量进行性下降 ,肝细胞钙含量呈进行性增多 ,与对照组相比差异有显著性意义 (P<0 .0 1) ,但BOI组上述变化较BO组明显 (P<0 .0 5 )。结论　胆红素钙结石成石过程中存在肝细胞膜钠泵活性的进行性下降 ,钠泵活性下降引起肝细胞钙超负荷及胆汁中胆汁酸含量下降 ,从而促进成石。细菌感染加重上述变化 。Objective To investigate the effects of activities of Na + K + ATPase in plasma membranes of hepatocytes on the formation of calcium bilirubinate gallstone. Methods The research were studied in Xiao's rabbit models.One hundred and three rabbits were randomly divided into the control group (Con, n =28),simple biliary obstruction group (BO, n =36),biliary obstruction and infection group (BOI, n =39).The activities of Na + K + ATPase,contents of intracellular calcium and bile acids in bile were measured in 3rd,7th,14th and 20th day. Results The activities of Na + K + ATPase,contents of bile acids in bile decreased gradually and the contents of intracelluar calcium of hepatocytes increased continously in all phases of experimental groups compared with control group ( P <0.01).There was more significant changes in BOI group than in BO group ( P <0.05). Conclusion The progressive decrease of activities of Na + K + ATPase has a close relationship with the over loaded intracellular calcium and the continous decrease of bile acids contents in bile in formation of calcium bilirubinate gallstone.Infection can promote above mentioned changes and more stone formation.

关 键 词：胆红素钙结石 钠泵 肝细胞钙 胆汁酸 兔 动物模型 

分 类 号：R657.4[医药卫生—外科学]