白细胞粘附分子在慢性阻塞性肺疾病及肺心病发病中作用的探讨  

Study of the function of leukocyte adhesion molecules in chronic respiratory diseases

作　　者：陈宇洁[1] 赵杰[1] 李德荣[1] 陆海英[1] 万小兵[1] 彭香[1] 雷松[2] 周东[2] 

机构地区：[1]四川省第四人民医院呼吸科,成都610016 [2]华西医科大学附属第一医院呼吸科

出　　处：《中华结核和呼吸杂志》2002年第2期94-97,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：四川省科委重点科学技术研究基金资助项目 ( 97 0 14 )

摘　　要：目的　探讨白细胞粘附分子在慢性阻塞性肺疾病 (COPD)、慢性肺原性心脏病 (肺心病 )发病机制中的作用 ,及抗白细胞粘附疗法在肺心病治疗中的疗效。方法　采用流式细胞术 ,用单克隆抗体定量测定 42例COPD患者 (稳定期 )、41例肺心病患者 (稳定期 )周围血中白细胞粘附分子CD11a、CD11b、CD18、CD54 的阳性表达率 ,并以 2 4名健康人作正常对照。41例肺心病患者分为 2组 ,治疗组 2 0例 ,服用火把花根片 (1次 3片 ,1日 3次 ) ;对照组 2 1例。测定 2 0例服火把花根片后的肺心病患者周围血中白细胞粘附分子CD11a、CD11b、CD18、CD54 的阳性表达率 ,并与对照组 2 1例肺心病患者作比较。结果　COPD患者组血液单核细胞表面的CD11a、CD11b、CD18、CD54 ,淋巴细胞表面CD11a的阳性表达率明显高于正常对照组 (P <0 0 5 ) ;肺心病患者组血液中性粒细胞表面CD11a、CD11b、CD18、CD54 ,单核细胞表面CD11a、CD18、CD54 ,淋巴细胞表面CD18较正常对照组明显增强 (P <0 0 5 ) ;服火把花根片后 ,肺心病治疗组中性粒细胞表面CD11a、CD11b、CD18、CD54 ,单核细胞表面CD11a、CD18、CD54 ,淋巴细胞表面CD18较服药前肺心病组有显著下降 (P <0 0 5 )。余各型均无显著差异。结论　COPD患者通过单核细胞粘附分子CD11a/CD18。Objective To study the function of leukocyte adhesion molecules in the pathogenesis of Chronic Obstructive Pulmonary Diseases(COPD) and cor pulmonale, and to evaluate the effect of anti-leukocyte-adhesion therapy for cor pulmonale Methods The positive expression rates of CD 11a ,CD 11b ,CD 18 ,CD 54 in peripheral blood were quantitatively analyzed by flow cytometry using monoclonal antibodies in 42 cases of COPD(stable stage),41 cases of cor pulmonale(stable stage),24 healthy controls as well as 20 cases of cor pulmonale who had taken huobahua Results The expression rates of CD 11a ,CD 11b ,CD 18 ,CD 54 in monocytes and CD 11a in lymphocytes from the COPD group were much higher than those in the control group( P <0 05) CD 11a ,CD 11b ,CD 18 ,CD 54 in neutrophils and CD 11a ,CD 18 ,CD 54 in monocytes, and CD 18 in lymphocytes in the cor pulmonale group were significantly stronger than those in the control group( P <0 05);whereas the positive expression of CDs was greatly reduced in the cor pulmonale patients who had taken huobahua( P <0 05) No differences were found in other groups Conclusions Upregulation of CD 11a /CD 18 ,CD 11b /CD 18 ,CD 54 in monocytes, CD 11a /CD 18 , CD 11b /CD 18 , CD 54 in neutrophils and CD 11a /CD 18 ,CD 54 in monocytes in cor pulmonale may promote adhesion of leukocytes with endothelial cells, which may play an important role in the pathogenic process Huobahua may prevent the development of cor pulmonale by downregulating the expression of CDs

关 键 词：白细胞粘附分子 慢性阻塞性肺疾病 肺心病 治疗 发病机制 

分 类 号：R563.9[医药卫生—呼吸系统]