复方丹参注射液对Ⅱ型胶原诱导性关节炎大鼠滑膜细胞分泌IL-1β的影响  

EFFECT OF COMPOUND DANSHEN INJECTION ON IL-1β SECRETION BY SYNOVIOCYTES IN COLLAGEN-Ⅱ INDUCED ARTHRITIS RATS

作　　者：黄清春[1] 张声鹏[2] 黄伟毅[1] 徐秋英[2] 陈光星[1] 沈鹰[1] 马伟[2] 

机构地区：[1]中国人民解放军广州军区广州总医院,广东广州510010 [2]广州中医药大学

出　　处：《安徽中医学院学报》2002年第2期50-52,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：广东省科委重点攻关项目 (99M0 4 4 0 8G)

摘　　要：目的 :研究复方丹参注射液对Ⅱ型胶原蛋白诱导性关节炎 (collagen Ⅱinducedarthritis ,CIA)大鼠模型滑膜细胞分泌IL 1β的影响。方法 :采用消化分离的方法获得滑膜细胞 ,放射免疫法检测大鼠滑膜细胞培养上清液中IL 1β的水平。结果 :复方丹参注射液可以明显下调CIA大鼠滑膜细胞分泌IL 1β的水平。结论 :复方丹参注射液治疗类风湿性关节炎的机制可能与下调滑膜细胞分泌IL 1β的水平有关。Objective:To study the effect of compound Danshen injection(CDI) on the secretion of IL 1β by synoviocytes in collagen Ⅱ induced arthritis(CIA) rats.Method:Synovium of knee joints from CIA rats was digested by collagenase and trypsin to prepare digested single synoval cell(DSSC).The content of the supernatant fluid was assayed by radioimmunoassay.Result:CDI could down regulate IL 1β production by DSSC.Conclusion:The mechanism for curative effect of CDI on arthritis is concerned with down regulating the IL 1β production by DSSC.

关 键 词：类风湿性关节炎 滑膜细胞 白细胞介素-1 复方丹参注射液 实验研究 

分 类 号：R259.932.2[医药卫生—中西医结合]