多巴酚丁胺对大鼠心室肌细胞Na^+/Ca^(2+)交换的影响及其受体机制  

Effect of dobutamine on Na^+/Ca^(2+) exchange and the involved receptor mechanisms in ventricular myocytes of rat

作　　者：韩清华[1] 武冬梅[2] 吕吉元[1] 吴博威[2] 

机构地区：[1]山西医科大学第一医院心内科,030001 [2]山西医科大学生理教研室

出　　处：《中华心血管病杂志》2002年第3期172-174,共3页Chinese Journal of Cardiology

基　　金：山西医科大学第一医院基金

摘　　要：目的　观察多巴酚丁胺对大鼠心室肌细胞Na+ /Ca2 + 交换电流的作用及其肾上腺素能受体机制。方法　用胶原酶消化的成年大鼠心室肌单个细胞及全细胞膜片钳技术 ,记录Na+ /Ca2 + 交换电流并观察药物对它的作用。结果　多巴酚丁胺呈剂量依赖性地增加大鼠心室肌细胞Na+ /Ca2 +交换电流 ,该作用可分别被 β和 β1受体阻断剂普萘洛尔和美托洛尔阻断。结论　多巴酚丁胺通过β1 受体途径增加大鼠心室肌细胞Na+ /Ca2 + 交换电流 ,这可能是多巴酚丁胺正性变力作用的一个新的机制。Objective To investigate the effect of dobutamine on Na +/Ca 2+ exchange current and the involved adrenoceptor mechanisms in ventricular myocytes of rat Methods Whole cell patch clamp technique was used to record the Na +/Ca 2+ exchange current and to observe its alteration induced by drugs Results Dobutamine increased the Na +/Ca 2+ exchange current with a dose dependent manner in ventricular myocytes of rat and this effect could be blocked by propranolol and metoprolol, respectively Conclusion The effect of dobutamine on Na +/Ca 2+ exchange current is mediated by β 1 receptor, and this effect may be a new mechanism underlying the positive inotropic action of dobutamine

关 键 词：多巴酚丁胺 离子交换 肾上腺能受体 膜片钳术 心室肌细胞 NA^+/CA^2+交换 

分 类 号：R541.6[医药卫生—心血管疾病]