人胎盘滋养层细胞表达白细胞介素-3  

Interleukin-3 expressed in human placental trophoblastic cells

作　　者：刘桂兰[1] 梁静[1] 贾月萍[1] 回允中[2] 虞有智[2] 

机构地区：[1]北京大学人民医院儿科,北京100034 [2]北京大学人民医院病理科,北京100034

出　　处：《北京大学学报（医学版）》2002年第1期80-82,共3页Journal of Peking University:Health Sciences

摘　　要：目的 :确定脐血中高含量白细胞介素 3(IL 3)的主要来源 ,进一步了解脐血造血微环境。方法 :应用抗IL 3多克隆抗体对 7例人工流产的绒毛标本和 1 0例健康产妇足月分娩的胎盘组织及脐带标本进行免疫组织化学染色。结果 :足月胎盘的滋养层细胞呈现阳性表达 ,绒毛标本的滋养层细胞未见阳性表达 ,在其他细胞和脐血管内皮细胞上未见阳性表达。结论 :胎盘滋养层细胞是脐血和胎盘表面的IL 3的主要来源。Objective:To investigate the cell secreting the interleukin 3 in human placenta and gain the knowledge of the hemotapoietic microenvironment of cord blood. Methods:Ten samples of term placenta tissue and umbilical cords were collected from ten health pregnant women, and other seven samples of villus were from seven artificial abortion cases. Samples were made into frozen sections and then analyzed by the techniques of immunohistochemical staining. Mouse antibody to IL 3 antigen was used as primary antibody, and biotinylated goat anti mouse antibody as secondary antibody. Alkaline phosphatase was stained and neutral red counterstained. Negative contrast was designed to be incubated without primary antibody. Results:After being stained, samples were observed under the microscope. Cells with blue contents in plasma were positive. Experiments indicated that the term placental trophoblastic cells had strong positive reactions while the samples of umbilical cord and villus showed negative reactions. Conclusion:Trophoblastic cells in the placental tissue are the main production source of IL 3. The secreting function of trophoblastic cells becomes mature with the growing of the embryo.

关 键 词：胎盘滋养层细胞 表达 白细胞介素3 

分 类 号：R714.56[医药卫生—妇产科学]