酶组织化学和免疫金显示大鼠胰淋巴管及其多肽激素转运的意义  

LYMPHATIC VESSELS AND ITS SIGNIFICANCE FOR TRANSPORTING POLYPEPTIDE HORMONES REVEALED BY OBSERVATION ON THE RAT PANCREAS WITH ENZYME-HISTOCHEMICAL STAINING AND IMMUNO-GOLD INJECTING

作　　者：王保芝[1] 王学礼[1] 王建钦[2] 王树峰[3] 王丽[4] 樊宇兵[1] 

机构地区：[1]河北医科大学基础医学院人体解剖学教研室,石家庄050017 [2]河北医科大学病理生理学教研室 [3]河北医科大学第二医院腺体外科 [4]河北医科大学电镜实验中心

出　　处：《河北医科大学学报》2002年第2期65-67,I001,共4页Journal of Hebei Medical University

基　　金：河北省卫生厅医学科学研究重点课题 (No .2K 0 17)

摘　　要：目的探讨胰腺内脉管系的分布特点及其多肽激素转运的意义。方法应用常规HE染色、5’ 核苷酸酶和碱性磷酸酶 (5’ Nase ALPase)双重组织化学染色、免疫金标记外源性胰岛素 (immuno goldmarkingexogenousinsulin ,IGMEI)注射技术 ,对大鼠胰组织冷冻切片进行了光镜 (LM )、扫描电镜 (SEM )二次电子和背散射电子图像(secondaryandbackscatteredelectronimages,SEI ,BEI)观察。 结果①经HE和 5’ Nase ALPase染色处理的胰组织切片LM观察表明 ,在大鼠胰腺内、较大的胰岛周围甚至内部 ,不仅存在血管 ,而且分布有丰富的淋巴管样结构 ;血管呈现ALPase强阳性蓝色反应 ,淋巴管表现 5’ Nase强阳性褐色反应。②免疫金标记胰岛素注射的胰组织切片SEM观察表明 ,SEI只显示胰腺的组织构造 ;BEI可显示标记金颗粒的背散射电子 ,主要存在于胰结缔组织间隙或组织通道、淋巴管或毛细淋巴管内。然而 ,胰腺的血管或毛细血管内 ,无背散射电子或BEI微弱。结论在较大的胰岛周围甚至内部 ,不仅存在血管 ,也分布有淋巴管 ;胰腺组织液的淋巴回流 ,对于胰岛素等多肽激素的正常转运 。ObjectiveTo explore the distributional feature of vascular system and its significance for transporting polypeptide hormones as insulin in the pancreas.MethodsUsing conventional HE, 5' nucleotidase and alkaline phosphatase (5' Nase ALPase) double histochemical staining methods, and a technique with immuno gold marking exogenous insulin (IGMEI) injecting into the pancreas, the cryostat sections of rat pancreas were observed with light microscope(LM)and scanning electron microscope (SEM) for secondary and backscattered electron images (SEI, BEI).Results①The LM observations of the cryostat sections of pancreas treated with HE and 5' Nase ALPase staining indicate that the lymphatic vessel like structure as well as blood vessels exist around even within the large pancreatic islets. The stronger ALPase blue reactions present in blood vessels, and the stronger 5' Nase brown reactions appear in lymphatic vessel like structure. ②The SEM observations of the cryostat sections of pancreas injected with IGMEI demonstrate that the SEI can be only showing the organizational feature of pancreas. However, BEI observations revealed the stronger backscattered electrons appear in the pancreatic connective tissue space or tissue channels, lymphatic vessels or capillaries. Nothing or weaker backscattered electrons present in blood vessels or capillaries. ConclusionThose lymphatic vessels as well as blood vessels distribute around even within the large pancreatic islets. The lymphatic drainage of pancreatic tissue fluid may play an important action for normal transporting of polypeptide hormones as insulin.

关 键 词：脉管 胰腺 酶组织化学法 免疫金标记技术 胰岛素 多肽激素转运 

分 类 号：R322.491[医药卫生—人体解剖和组织胚胎学]