离子色谱荧光检测植物生长调节素吲哚-3-乙酸和吲哚-3-丁酸  

Determination of Trace of Indoleacetic acid and lndolebutyric acid with ion chromatography and fluorescene detection

作　　者：施青红[1] 吴东亮 陈志斌 叶明立[1] 李明 凌艳艳[1] 朱岩[1] 

机构地区：[1]浙江大学西溪化学系,杭州310028

出　　处：《宁波高等专科学校学报》2001年第B03期147-150,共4页Journal of Ningbo College

基　　金：浙江省自然科学基金;浙江省分析测试基金资助项目

摘　　要：吲哚 -3 -乙酸和吲哚 -3 -丁酸在离子色谱仪Dionex 4 0 0 0i上使用荧光检测器进行检测。该测定采用 1 .0ml/min的 0 .4 5V/VCH3OH ,0 .0 1mol/lNa2 CO3的混合溶液作为淋洗液在DinoexOmniPacPAX -50 0柱分离。吲哚 -3 -乙酸和吲哚 -3 -丁酸的检测限分别为 0 .0 4 5μg/ml和 0 .1 67μg/ml,有良好的重现性和线性关系。对土壤样品进行测定 ,有较好的回收率。indoleacetic acid and indolebutyric acid in soil are detected by Dionex 4000i ion chromatography with fluorescence detector. This experiment is performed by OmniPac PAX-500 column using 0.010mol/L Na 2CO 3, 0.45V/V CH 3OH as eluate.The fluorescene detector was measured in excitation and emission wavelengths of 285nm ,360nm,respectively.The detecting limit is 0.045μg/mL和0.167μg/mL for indoleacetic acid and indolebutyric acid,respectively, The method with linering、 selection and sensitivity can be used in trace amount indoleacetic acid and indolebutyric acid in soil samples.

关 键 词：吲哚-3-乙酸 吲哚-3-丁酸 离子色谱法 荧光检测器 植物生长调节素 含量测定 

分 类 号：TQ452[化学工程—农药化工] TQ450.7