冬虫夏草和中国拟青霉对细胞免疫功能的影响  

Effect of Cordyceps Sinensis (CS) and Paecilomyces Sinensis (PS) on Immune Function in Mice

作　　者：张传开[1] 袁盛榕[1] 刘进学[1] 

机构地区：[1]首都医科大学药理教研室

出　　处：《中药药理与临床》1998年第3期21-23,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：本文采用正常、环磷酰胺（Ｃｙ）诱导增强和降低的ＤＮＦＢ性迟发型超敏反应（ＤＴＨ）模型以及丝裂原ＣｏｎＡ、ＬＰＳ诱导的淋巴细胞转化的方法，比较研究了冬虫夏草（ＣＳ）和中国拟青霉（ＰＳ）对机体细胞免疫功能的影响。结果表明：ＣＳ（０．５、１．０ｇ／ｋｇ·ｄ×７ｄ）和ＰＳ（１．０、２．０、４．０ｇ／ｋｇ·ｄ×７ｄ）对正常小鼠ＤＴＨ反应无明显影响，但ＣＳ（２．０ｇ／ｋｇ·ｄ×７ｄ）和ＰＳ（４．０ｇ／ｋｇ·ｄ×７ｄ）均可使增高或降低的ＤＴＨ恢复到正常水平（Ｐ＜０．０１）。在体外，ＣＳ（０．２４４～７．８ｍｇ／ｍｌ）和ＰＳ（０．４８８～３．９ｍｇ／ｍｌ）均可明显提高ＣｏｎＡ活化的淋巴细胞转化反应（Ｐ＜０．０１），并均在０．９７５ｍｇ／ｍｌ时作用达到高峰：ＣＳ（０．２４４～７．８ｍｇ／ｍｌ）和ＰＳ（０．１２２～７．８ｍｇ／ｍｌ）均可显著增强ＬＰＳ诱导的淋巴细胞转化反应（Ｐ＜０．０１），并呈剂量依赖性，在１．９５ｍｇ／ｍｌ时作用达到高峰。由此可见。 Influence of CS and PS on celllular immunity in mice was comparatively studied.DNFB induced delayed type hypersensitivity (DTH) combined with enhanced or suppressed DTH model established by ip cyclophosphamide (Cy),and suboptimal mitogen (ConA,LPS) induced lymphocyte transformation were employed in vivo/vitro,respectively.The results indicated that CS(0.5,1.0g/kg·d×7d) and PS((1.0,2.0,4.0g/kg·d×7d) hadn′t any influence on DTH reaction,however,CS(2.0g/kg·d×7d) and PS(4.0g/kg·d×7d) thoroughly restored over magnified or attenuated DTH reaction induced by Cy (P<0.01).In vitro assay,both CS(0.244～7.8mg/ml) and PS(0.488～3.9mg/ml) significantly enhanced ConAinduced lymphocyte transformation (P<0.01),the optimal concentration was 0.975mg/ml;CS(0.244～7.8mg/ml) and PS(0.122～7.8mg/ml) markedly elevated lymphocyte transformation induced by LPS in a concentration dependent manner (P<0.01),1.95mg/ml was the optimal dose.In conclusion,both CS and PS regulated cellular immune function in mice.

关 键 词：冬虫夏草 中国拟青霉 细胞免疫功能 迟发性超敏反应 淋巴细胞转化 

分 类 号：R282.71[医药卫生—中药学]