灵芝多糖对小鼠脾细胞白细胞介素1、肿瘤坏死因子的产生及其mRNA表达的影响  

Effects of Ganoderma polysaccharide on production and mRNA expressions of IL1α and TNFα in murine splenocytes in vitro

作　　者：雷林生[1] 王庆彪[1] 孙莉莎[1] 杨淑琴[1] 

机构地区：[1]第一军医大学药理学教研室

出　　处：《中药药理与临床》1998年第2期16-18,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金

摘　　要：小鼠脾细胞体外培养３ｈ即可检测到ＩＬ１αｍＲＮＡ的表达，４ｈ达到高峰，５ｈ后降至不能检出的水平。ＴＮＦαｍＲＮＡ在４ｈ开始出现，４．５ｈ时达到高峰，５．５ｈ后逐渐消失。在培养开始时分别加入灵芝多糖ＧＬＢ７５、１０、２０、４０μｇ／ｍｌ，４ｈ及４．５ｈ分别检测ＩＬ１α及ＩＬ１αｍＲＮＡ的表达情况。结果ＧＬＢ７能促进ＩＬ１α及ＩＬ１αｍＲＮＡ的表达，对ＩＬ１αｍＲＮＡ的表达提高率分别为７．６％、６７．３％、１０５．０％、１４３．１％；对ＴＮＦαｍＲＮＡ的表达提高率分别为９．９％、３３．９％、４５．９％、７５．８％。８ｈ后ＧＬＢ７对培养上清中ＩＬ１活性的提高率分别为１．４％、３．０％、６．７％、８．１％；１２ｈ后对培养上清中ＴＮＦα活性的提高率分别为３．０％、１４．１％、２８．２％、３５．７％。结果说明灵芝多糖ＧＬＢ７能通过促进小鼠脾细胞原代培养时ＩＬ１α及ＴＮＦαｍＲＮＡ的表达从而促进ＩＬ１及ＴＮＦα的合成与分泌。Interleukin1(IL1)α mRNA expression could be detected in murine splenocytes after 3h incubation,it reached the peak level at 4h and then declined to be undetected after 5h incubation.TNF(Tumor Necrosis Factor)α mRNA expression occured after 4h incubation,reached the peak level at 4.5h and disappeared gradually after 5.5h.Ganoderma polysaccharide (GLB7) in different final concentrations(5,10,20,40μg/ml) was added into the cultures at the beginning of incubation and then,the amount of mRNA expressions for IL1α and TNFα was determined after 4h and 4.5h respectively.It was found that GLB7 could promote the mRNA expressions for both IL1α and TNFα,the increase rates of mRNA expression for IL1α were 7.6%,67.3%,105.0% and 143.1%,and were for TNFα 9.9%,33.9%,45.9% and 75.8% respectively.After 8h incubation,GLB7 could increase the activity of IL1 in the supernatants by 1.4%,3.0%,6.7% and 8.1%,and raise the activity of TNFα by 3.0%,14.1%,28.2% and 35.7% after 12h incubation respectively.It is suggested that the enhancement effects of Ganoderma polysaccharide on the production and secretion of IL1 and TNFα in primary murine splenocyte culture are due to promoting of the mRNA expressions for IL1α and TNFα respectively.

关 键 词：灵芝多糖 白细胞介素I 肿瘤坏死因子 mRNA 抗肿瘤作用 免疫调节 

分 类 号：R286.91[医药卫生—中药学] R285[医药卫生—中医学]