补阳还五汤及其有效部位对沙鼠脑缺血后DND脑能量代谢、NO和NOS的影响  

Effect of Bu Yang Huan Wu Tang on Cerebral Energy Metabolism, Nitric Oxide and Nitric Oxide Synthase During Delayed Neuronal Death Induced by Cerebral Ischemia in Gerbils

作　　者：邓常青[1] 邓奕晖[1] 向华林[1] 贺福元[1] 唐映红[2] 

机构地区：[1]湖南中医学院,长沙410007 [2]湖南医药工业研究所,410014

出　　处：《中药药理与临床》1999年第5期3-6,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金

摘　　要：目的：比较研究补阳还五汤和其有效部位组方抗脑缺血后ＤＮＤ 作用与脑组织能量代谢、ＮＯ 和ＮＯＳ 的关系。方法：采用沙鼠双侧颈总动脉夹闭脑缺血再灌注模型，于再灌注后４８ 小时取前脑皮质测定Ｎａ ＋Ｋ＋ＡＴＰ 酶、Ｃａ２ ＋ＡＴＰ 酶、Ｍｇ２ ＋ＡＴＰ 酶、乳酸、ＮＯ 和ＮＯＳ。结果：缺血再灌注后４８ 小时脑组织Ｎａ＋Ｋ＋ＡＴＰ酶、Ｃａ２ ＋ＡＴＰ 酶及Ｍｇ２ ＋ＡＴＰ 酶活性均降低，ＮＯ 含量和ＮＯＳ 活性降低，而乳酸含量无明显变化。补阳还五汤和有效部位组方ｉｐ 均可防止缺血再灌注４８ 小时后Ｎａ＋Ｋ＋ＡＴＰ 酶活性的降低，增加ＮＯ 含量，提高ＮＯＳ 活性，而有效部位组方还可增加缺血再灌注后Ｃａ２ ＋ＡＴＰ 酶和 Ｍｇ２ ＋ＡＴＰ 酶活性，且其升高ＮＯ 的作用强于补阳还五汤组。结论：脑缺血后迟发性神经元坏死与脑内能量代谢障碍和ＮＯ 的生成减少有一定的关系，补阳还五汤及其有效部位组方可能通过调节脑内能量代谢和ＮＯObjective: Effect of Bu Yang Huan Wu Tang (BYHWT) and its available composition combination antagonisming delayed neuronal death (DND) induced by cerebral ischemia and relation between DND and cerebral tissue energy metabolism, nitric oxide (NO) and nitric oxide synthase (NOS) will be studied. Method: cerebral ischemia model was made by ligating bilateral arteries carotis communis for 20 min followed reperfusion 48h in gerbils. Na + K + ATPase activity, lactic acid content, Ca 2+ ATPase, Mg 2+ ATPase activity, NO content and NOS activity were determined in fore brain cortex at 48h after reperfusion. Result: Na + K + ATPase, Ca 2+ ATPase and Mg 2+ ATPase activity lowered (P<0.01), NO level decreased (P<0.01), NOS activity dropped (P<0.01) in cerebral tissue at 48h after reperfusion, but lactic acid level had no change. BYHWT could prevent reduction of Na + K + ATPase activity (P<0.05, P<0.01), increase NO level (P<0.01), enhance NOS activity (P<0.01) at 48h after reperfusion. Conclution: DND induced by ischemia may be concerned with energy metabolism disorder and decrease of NO formation, BYHWT may play the role of antagonisming cerebral ischemia by adjusting cerebral energy metabolism and nitric oxide formation.

关 键 词：补阳还五汤 脑缺血 ATP酶 一氧化氮 一氧化氮合酶 迟发性神经元坏死 

分 类 号：R289.5[医药卫生—方剂学] R277.733[医药卫生—中药学]