银杏叶提取物对坐骨神经损伤后运动神经元超微结构的影响  

Influence of Ginkgo Biloba Extract on Ultrastructural Changes of Spinal Motorneurons Following Sciatic Nerve Injury in Rats

作　　者：薛锋[1] 顾玉东[1] 陈德松[1] 李继峰[1] 钟慈声 

机构地区：[1]复旦大学附属华山医院手外科,上海200040 [2]医学院生物物理教研室,上海200032

出　　处：《复旦学报（医学版）》2002年第2期138-141,共4页Fudan University Journal of Medical Sciences

基　　金：国家重点基础研究项目 ( 973)资助课题 (NoG19990 5 42 0 2 );卫生部重点学科课题基金资助课题 (NoD963-3)

摘　　要：目的　研究银杏叶提取物 (EGb761)对大鼠坐骨神经损伤后脊髓前角运动细胞超微结构的影响。方法　SD大鼠 18只 ,随机分成两组 ,银杏叶提取物 (EGb761)组和生理盐水 (SAL)组。切断大鼠坐骨神经并将神经远近端分别反折结扎 ,术后经腹腔分别注射EGb761( 10 0mg (kg·d)和同体积的SAL ,于 2、4、6周取材L4～ 6 节段脊髓 ,电镜下观察前角大型运动神经元细胞核及细胞器的超微结构形态变化 ,用体视学方法计算每张照片线粒体、内质网及溶酶体面积密度变化。结果　两组脊髓前角大型运动神经元超微结构均发生损伤性改变 ,实验组超微结构形态以及线粒体、内质网及溶酶体体积密度变化程度明显好于对照组。结论　银杏叶提取物能减轻大鼠坐骨神经损伤后前角运动神经元超微结构的损伤性改变 。Purpose: To investigate the protective effect of Ginkgo biloba extract (EGb761) on ultrastructural changes of spinal motomeurons after peripheral nerve injury. Methods: Model of sciatic nerve severance and end ligation was made in 18 SD rats. Two groups were divided. One for EGb761 injection [100 mg/(kg&middotd)], another for 0.9% saline. L4-6 spinal cord were harvested and electron miroscope study of motorneurons' ultrastracture was conducted at 2nd, 4th and 6th week postoperatively. Morphological observation and volume density of rough endoplasmic reticulum mitochondrion, and lysosome were made. Results: Ultrastructural changes occurred in Anterior horn motorneurons after axonotomy. Compared with control group, ultrastructural changes of spinal motoneurons in experimental group were little. Conclusions: EGb761 might has certain protective effect on spinal motoneuron after peripheral nerve injury.

关 键 词：周围神经 坐骨神经损伤 超微结构 银杏叶提取物 体视学 影响 

分 类 号：R651.3[医药卫生—外科学] R285.5[医药卫生—临床医学]