炎症细胞因子参与新生鼠窒息后肾损伤的作用时间  

Research on the time-effect relationship of inflammatory cytokines in post-asphyxial renal injury in neonatal rats

作　　者：董文斌[1] 唐章华[1] 翟雪松[1] 邹艳[1] 陈书琴[1] 陈红英[1] 龙汉安[2] 陈跃[3] 华丽[1] 

机构地区：[1]泸州医学院附属医院儿科,泸州医学院肾功能保护研究室,四川泸州646000 [2]泸州医学院附属医院病理科,四川泸州646000 [3]泸州医学院附属医院核医学科,四川泸州646000

出　　处：《中华急诊医学杂志》2002年第1期25-26,共2页Chinese Journal of Emergency Medicine

基　　金：四川省卫生厅科研基金 (NO .980 17)

摘　　要：目的　研究炎症细胞因子参与窒息后肾损伤的作用高峰时间。方法　将 45只 7～ 10d龄Witsar大白鼠随机分为四组 ,即对照组 (13只 )、窒息后复氧 2h组 (10只 )、窒息后复氧 2 4h组 (11只 )、窒息后复氧 48h组 (10只 )。制成常压窒息模型。在上述各时相点取左肾称重求出左肾系数 (LRC) ,并分别采用放射免疫分析法 (RIA)和双抗体夹心ELISA ABC法检测肾组织白细胞介素 1、白细胞介素 8(IL 8)及白细胞介素 6 (IL 6 )的含量 ,同时在光镜下对肾小管损伤程度进行评分 ,并计数肾微血管床的白细胞滞留数。结果　窒息后复氧 2h开始 ,IL 1、IL 8、IL 6水平及白细胞滞留数升高 ,在 2 4h时达到高峰 ;同时左肾系数、肾小管评分也呈一致的改变。结论　窒息后肾组织炎性细胞因子参与肾损伤的高峰时间为 2 4h。Objective To determine the time effect relationship of inflammatory cytokines in post asphyxial renal injury in neonatal rats.Methods 45 neonatal Wistar rats of 7～10 days old were randomly divided into 4 groups:the control(n=13)?2 h post asphyxia(n=10),24 h post-asphyxia(n=11) and 48 h post-asphyxia(n=10).The experiment groups were established by normobaric asphyxia.Changes in left renal coefficient(LRC),leukocyte count,the scores of tubules and its ultrastructure were observed.The contents of interleukin 1(IL 1),interleukin 8(IL 8),interleukin 6(IL 6) of renal tissue were measured.Results Compared with the control,leukocyte,LRC,the scores of tubules,IL 1,IL 8 and IL 6 began to increase from 2 h post asphyxia and reached the peak at 24 h post asphyxia.Conclusion Our results suggeste that release of inflammatory cytokines may play an importmant role in the development of post asphyxial renal injury,and its maximal effect is at 24 h post asphyxia. [

关 键 词：窒息 肾损伤 炎症细胞因子 新生鼠 

分 类 号：R722.12[医药卫生—儿科] R726.92[医药卫生—临床医学]