渗透胁迫对玉米内源ABA及PMH^+-ATPase活性影响  

The Influence of Water Stress on Endogenous ABA and PM H^+-ATPase Activity of the Growth Zone Cell in Zea Mays L.

作　　者：胡章立[1] 

机构地区：[1]深圳大学生物工程系,深圳518060

出　　处：《深圳大学学报（理工版）》2002年第1期54-59,共6页Journal of Shenzhen University(Science and Engineering)

摘　　要：用 -1 0MPa的PEG Hoagland溶液对抗旱性较强的陕 841 3和抗旱性较差的陕 841 0玉米品种进行胁迫处理 ,结果发现 ,胁迫处理会导致细胞内源脱落酸 (ABA)含量和质膜 (PM)H+ ATPase活性发生明显的变化 ;无论是胁迫还是对照 ,陕 841 3玉米幼叶生长部位细胞内源ABA含量和PMH+ ATPase活性均高于陕 841 0 ,但在胁迫条件下 ,这种差异更明显 .对玉米幼苗 2 4h连续胁迫处理 ,在多数情况下 ,细胞内源ABA含量和PMH+ ATPase活性维持在一般水平 (但较对照高 ) ,且两者之间没有发现直接的相关性 ;但在胁迫 2h处 ,细胞内源ABA含量出现峰值 ,此时PMH+ ATPase活性则在较低的水平 ;在胁迫 4h处 ,PMH+ ATPase活性表现出很高的峰值 ,而此时细胞内源ABA水平则相对较低 ,两者在各自峰值处存在一定的负相关 .The roots of intact maize seedling of two species (including Shaan 8 413 with high drought tolerance and Shaan 8 410 with low drought tolerance) were treated by PEG Hoaglands solution with an osmotic potential of -1 0Mpa, the endogenous ABA content and PM H + ATPase activity of cells in two species were stimulated markedly. It was found by comparison that the endogenous ABA content and PM H + ATPase activity were much higher in Shaan 8 413 than those in Shaan 8 410 under the treatment and control. However, the difference between Shaan 8 413 and Shaan 8 410 was more obvious under treatment condition than that under control. The maize seedling was treated by continuous stress for 24 h and samples were taken for detecting the endogenous ABA content and PM H + ATPase activity at zero time and at different duration. The endogenous ABA content and PM H + ATPase activity were maintained at a normal level(higher than control). In most cases, no relationship was found between the endogenous ABA content and PM H + ATPase activity. However, when the seedling was treated for 2 h, the endogenous ABA content of cell reached the peak, and PM H + ATPase activity remained at a normal level; when the seedling was treated for 4 h, the PM H + ATPase activity of cell reached a peak value, but the endogenous ABA content still kept at a low level. It was indicated that there was a negative relationship between the endogenous ABA content and PM H + ATPase activity in maize cell at their respective peak values.

关 键 词：内源ABA PMH^+-ATPase活性 渗透胁迫 抗旱机理 水分胁迫 内源脱落酸 

分 类 号：S311[农业科学—作物栽培与耕作技术] Q945.78[农业科学—农艺学]