低硒鼠模型的复制  

REPRODUCING OF SELENIUM-DEFICIENT MOUSE MODEL

作　　者：张淑芹[1] 赵林山[1] 郑继奎[1] 杨永良[1] 钟学宽[2] 郭立波[3] 

机构地区：[1]哈尔滨医科大学动物学部 [2]黑龙江省克山病研究所 [3]哈尔滨师范大学

出　　处：《哈尔滨师范大学自然科学学报》2002年第1期88-91,共4页Natural Science Journal of Harbin Normal University

摘　　要：本文研究硒缺乏对动物心肌损伤的影响，借以探讨硒缺乏和克山病的关系．采用低硒的合成饲料喂养五周龄ＢＡＬＢ／Ｃ雄性小鼠五周，测定全血中的谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性及肝脏中硒的含量，用光镜和电镜观察心肌的病变．实验结果表明缺硒组小鼠全血中ＧＳＨ－Ｐｘ活性及肝硒值明显低于对照组（Ｐ＜０．０１），成功地复制出缺硒动物模型．In this paper, the effect of selenlum deficiency on myocardial injuries and the r elationship between selenium deficiency and Keshan Disease were investigated.Mal e BALB/C mice fed with synthesized low-selenium food,which were five years old,w ere raised for 5 weeks.The GSH-Px activity in the whole blood and the selenium l evel in the livers of the mice were detected.The disease changes in myocardial m uscles were observed by using light microscope and electronic microscope.The res ults indicated tha t the livers of selenium-deficient mice were significantly lower than that the c o ntrolled (P<0.01).The selenium-deficient mouse model was successfully reproducib le according to protocol in the study.

关 键 词：BALB/C小鼠 缺硒动物模型 心肌损伤 克山病 发病机理 谷胱甘肽过氧化物酶活性 

分 类 号：R542.3[医药卫生—心血管疾病] R－332[医药卫生—内科学]