东北地区3株猪细小病毒7型毒株基因组测定及遗传进化分析  

Gene Sequencing and Genetic Evolution Analysis of Three PPV7 Strains in Northeast China

作　　者：张涵 解长占[1,3] 李太元 哈卓[1] 李卓昕 李成辉 李金凤 赵冠宇[1,4] 郭影成 鲁会军[1] 金宁一 Zhang Han;Xie Changzhan;Li Taiyuan;Ha Zhuo;Li Zhuoxin;Li Chenghui;Li Jinfeng;Zhao Guanyu;Guo Yingcheng;Lu Huijun;Jin Ningyi(Institute of MilitaryVeterinary Medicine, The Academy of Military Medical Sciences, Changchun, Jilin 130122, China;College of Agriculture, YanbianUniversity, Yanbian, Jilin 133002, China;College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China;College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China;Fengman District Animal Disease Prevention and Control Center of Jilin City, Jilin, Jilin 132013, China:)

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130122 [2]延边大学农学院,吉林延边133002 [3]吉林农业大学动物科学技术学院,吉林长春130118 [4]吉林大学动物医学学院,吉林长春1300625 [5]吉林市丰满区动物疫病预防控制中心,吉林吉林132013

出　　处：《中国动物检疫》2018年第11期71-74,共4页China Animal Health Inspection

基　　金：国家重点研发计划项目(2016YFD0500401;2018YFD0500104)

摘　　要：从东北地区采集的150份猪肺脏样品中,检测出3株猪细小病毒毒株。根据PPV7标准株NCBI登录号KU563733设计3对引物,对3个病毒株的基因片段进行扩增测序,将测序结果依次进行拼接,获得3个病毒株的NS1和CP基因序列,然后进行序列的同源性比对及进化树分析。结果显示:3个毒株的NS1基因序列与经典PPV7毒株的同源性分别为94.4%～95.2%、94.1%～95.1%和93.5%～94.3%;CP基因序列与经典PPV7毒株的同源性分别为89.6%～91.8%、89.8%～92.0%和89.6%～91.7%。通过进化树比对分析,确认3株PPV毒株为PPV7亚型。通过对其他病毒进行检测,发现PPV7与PCV2、PPV2的混合感染率较高。该分析结果为我国东北地区猪病防控提供了依据。Recently, Three strains of porcine parvovirus (PPV) were isolated from 150 pig lung samples collected from northeast China. Based on the reference sequence of PPV7 standard strain published in NCBI (accession number: KU563733), three pairs of primers were designed, and the gene fragments of the three virus strains were amplified and sequenced. After sequence assembly, the whole sequences of NS1 and CP gene of the three strains were obtained. At last, the sequence homology was compared, and evolutionary tree was drawn. The results showed that the sequence homologies of the NS1 gene between the 3 strains and the classical PPV7 strain were 94.4% to 95.2%, 94.1% to 95.1% and 93.5% to 94.3%, respectively. The homologies of the CP gene between the 3 strains and the classical PPV7 strain were 89.6% to 91.8%, 89.8% to 92.0% and 89.6% to 91.7%, respectively. According to the phylogenetic analysis, the 3 strains were all identified as PPV7 subtype. Through detecting other viruses, it was found that the mixed infection of PPV7, PCV2 and PPV2 was relatively. The analysis results would provide a basis for prevention and control of swine diseases in northeast China.

关 键 词：猪细小病毒7型(PPV7) 基因组测定 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]