氢溴酸樟柳碱对离体大鼠颈总动脉的作用及机制研究  

In vitro Effect and Mechanism of Anisodine Hydrobromide on Common Carotid Artery of Rats

作　　者：潘媛[1] 彭成[1] 江虹雨 岳美颖 唐光梅 谢晓芳[1] PAN Yuan;PENG Cheng;JIANG Hongyu;YUE Meiying;TANG Guangmei;XIE Xiaofang(College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China)

机构地区：[1]成都中医药大学药学院,成都610075

出　　处：《四川动物》2018年第6期660-666,共7页Sichuan Journal of Zoology

基　　金：四川省科技支撑计划(2016SZ0027)

摘　　要：目的观察氢溴酸樟柳碱对离体大鼠颈总动脉的作用及相关机制。方法麻醉大鼠后,分离得到大鼠颈总动脉并制成血管环,采用离体血管环实验,观察氢溴酸樟柳碱在1×10^(-4)～5×10^(-3)mol·L^(-1)浓度范围内对KCl、苯肾上腺素(PHE)预收缩的内皮完整及去内皮血管环的作用;并观察预孵一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L-NAME)、不同的钾离子通道抑制剂格列本脲(Gly)、4-氨基吡啶(4-AP)、四乙基氯化铵(TEA)、BaCl_2对氢溴酸樟柳碱舒张血管环作用的影响;以2×10^(-3)mol·L^(-1)氢溴酸樟柳碱预孵血管环,观察其对以细胞内、外钙为收缩剂的血管收缩的作用,并探讨其舒张血管的机制。结果氢溴酸樟柳碱在体外1×10^(-4)～5×10^(-3)mol·L^(-1)浓度范围内能浓度依赖性舒张KCl和PHE预收缩的血管环,对KCl预收缩的血管环最大舒张幅度(Emax)为33. 97%±11. 53%,并在低浓度(1×10^(-4)～1×10^(-3)mol·L^(-1))收缩血管(P <0. 01,P <0. 05),对PHE预收缩的血管环的半数有效浓度为5. 61(3. 88,8. 10) mmol·L^(-1),Emax=47. 93%±18. 63%;对PHE预收缩的去内皮血管环,氢溴酸樟柳碱舒张血管的Emax无明显变化;而L-NAME、Gly、4-AP、TEA、BaCl_2对氢溴酸樟柳碱舒张PHE预收缩的血管环均无明显作用;在无Ca^(2+)溶液中,2×10^(-3)mol·L^(-1)氢溴酸樟柳碱可以显著增强PHE引起的血管环短暂收缩(P <0. 01)。结论氢溴酸樟柳碱能够在低浓度收缩离体大鼠颈总动脉环,并能浓度依赖性地舒张离体大鼠颈总动脉环,对血管的张力具有双向作用,且其机制与非内皮依赖途径及促肌浆网内钙释放相关。Objective To study the effect and mechanism of anisodine hydrobromide on common carotid artery of rats. Methods The common carotid artery of anesthetized rat was isolated and made into a vascular ring. Potassium chloride (KC1) or phenylephrine (PHE) precontraeted rat common carotid artery was then treated by anisodine hydrobromide at a concentration range of 1 x 10-4-5 x 10-3 mol.L-l. The nitric oxide synthase inhibitor N^-nitro-L-arginine methyl ester (L-NAME) or potsssium ( K+) channels inhibitors Glyburide ( Gly), 4-Aminopyridine (4-AP), Tetraethylammonium chloride (TEA) and BaC12 were used to study their influence on the anisodine hydrobromide-induced vasorelaxation. Intra- cellular or extracellular calcium ( Ca2 ~ ) was used as agonist to deterimine whether contractile response could be affected by anisodine hydrobromide at a concentration of 2 x 10-3 mol ~ L-1. Results The constricted common carotid artery ring in- duced by KC1 or PHE could be dilated by anisodine hydrobromide in a concentration range of 1 x 10-4 -5 x 10-3 mol ~ L-~ in vitro. In common carotid artery rings precontracted by KC1, anisodine hydrobromide-induced maximal relaxation magni- tude (Em^x) was 33.97% + 11.53%, and a vasoconstrictor function was observed in the concentration of 1 x 10-4-1 x 10-3 mol ~ L-1 (P <0. 01, P <0. 05). By contrast, in aortic rings precontracted by PHE, anisodine hydrobromide-in- duced median effect concentration (EC50) was 5.61 (3.88, 8. 10) mmol ~ L-1, and Era~ was 47. 93 % _+ 18.63%. Com- pared with the endothelial complete blood vessel ring, there was no significant change in the Era= of anisodine hydrobromide in the de-endothelial common carotid ring pre-contracted by PHE. Moreover, the supplementation of nitric oxide synthase inhibitor L-NAME or K + channels inhibitors Gly, 4-AP, TEA and BaC12 had no significant effect on anisodine hydrobro- mide-indueed vasorelaxation. In the absence of Ca2 + solution, PHE-induced transient vasoconstriction could be significantly enhanced by anisodine hydrobromide in a co

关 键 词：氢溴酸樟柳碱 血管环 收缩 舒张 

分 类 号：Q95-33[生物学—动物学]