雄黄与亚砷酸钠亚急性暴露对小鼠砷代谢及氧化应激影响的比较  

Comparison of effects of realgar and sodium arsenite on arsenic metabolism and oxidative stress in mice

作　　者：王达[1] 李永芳[1] 林琳[2] 李冰[1] 孙贵范[1] WANG Da;LI Yong-fang;LIN Lin;LI Bing;SUN Gui-fan(College of Public Health,China Medical University,Shenyang,Liaoning 110122,China)

机构地区：[1]中国医科大学公共卫生学院,辽宁沈阳110122 [2]中国医科大学基础医学院

出　　处：《环境与健康杂志》2018年第6期480-482,共3页Journal of Environment and Health

基　　金：国家自然基金青年项目(81502764)

摘　　要：目的探讨经口染毒雄黄、亚砷酸钠后,小鼠体内砷代谢及氧化应激指标的变化,为评价雄黄的毒性提供实验数据。方法清洁级雌性昆明小鼠随机分为对照组(0.5%羧甲基纤维素钠)、0.5 g/kg雄黄组和0.1 g/kg亚砷酸钠组,各组均灌胃染毒3 d,用氢化物发生-超低温捕集-原子吸收分光光度法分别测定尿液和肝组织中无机砷(iAs)、一甲基胂(MMA)和二甲基胂(DMA)。用试剂盒法测定全血中GSH和肝脏T-AOC水平。结果雄黄组小鼠尿液和肝脏中各形态砷和总砷含量高于对照组,但低于亚砷酸钠组;一甲基化率(FMR)和二甲基化率(SMR)高于亚砷酸钠组,差异均有统计学意义(P<0.05)。雄黄组全血GSH和肝脏T-AOC水平均高于亚砷酸钠组(P<0.05),其全血GSH和肝脏T-AOC水平与对照组相比差异均无统计学意义(P>0.05)。结论与亚砷酸钠相比,雄黄中的砷在小鼠体内代谢较慢,对氧化应激指标影响较小。Objective To investigate the changes of arsenic metabolism in mice and its effects on oxidative stress after oral administration of realgar or sodium arsenite,provide experimental data for the evaluation of the toxicity of realgar. Methods The clean grade female Kunming mice was divided into the control group (0.5% sodium carboxymethyl cellulose), the realgar group (0.5 g/kg) and the sodium arsenite group (0.1 g/kg). Each group was given intragastric administration for three days. Hydride generation-cryogenic capture-atomic absorption spectrophotometry were used to determine the levels of inorganic arsenic (iAs), monomethyl arsenic (MAA) and dimethyl arsenic (DMA) in 24-h urine samples and liver tissue respectively. Whole-blood GSH and liver T-AOC levels were determined by kit method. Results In realgar group,the concentrations of arsenic species and total arsenic in urine and liver were significantly higher than those of control group,and lower than those of sodium arsenite group;The levels of first methylation ratio (FMR) and second methylation ratio (SMR) were significantly higher than those of sodium arsenite group (P<0.05) ; Blood GSH and liver T-AOC levels were significantly higher than those of sodium arsenite group(P<0.05), and revealed no significantly differences compared with those of control group(P>0.05). Conclusion Arsenic in realgar presents lower metabolism, and less impact on the indicators of oxidative stress in mice.

关 键 词：雄黄 亚砷酸钠 砷代谢 氧化应激 小鼠 

分 类 号：R994.6[医药卫生—毒理学]