基于单核苷酸多态性鉴别小梅山猪及其制品  

Identification of Small Meishan Pig(Sus scrofa) and Its Products Based on Single Nucleotide Polymorphism

作　　者：岳阳 张哲[1] 徐忠 孙浩[1] 刘登英 赵伟 马裴裴 张向喆[1] 王起山[1] 潘玉春[1] YUE Yang;ZHANG Zhe;XU Zhong;SUN Hao;LIU Deng-Ying;ZHAO Wei;MA Pei-Pei;ZHANG Xiang-Zhe;WANG Qi-Shan;PAN Yu-Chun(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 222000,China)

机构地区：[1]上海交通大学农业与生物学院,上海222000

出　　处：《农业生物技术学报》2018年第12期2168-2175,共8页Journal of Agricultural Biotechnology

基　　金：上海市农委项目(沪农科引字(2017)第1-1号和沪农科推字(2016)第1-1-4号);国家自然科学基金(No.31772552)

摘　　要：小梅山猪(Sus scrofa)是太湖流域独特的地方猪品种,具有肉质鲜美等特点,市场上存在小梅山猪及其肉制品的假冒问题,而传统鉴别技术在实际应用中具有一定的局限性。本研究利用第3代分子标记—单核苷酸多态性(single nucleotide polymorphism, SNP)开发小梅山猪及其制品的分子标记鉴定方法,将小梅山猪与太湖流域其他6个品种及常见西方品种猪进行鉴别区分。对于太湖流域7个品种(品系)(中梅山猪、小梅山猪、二花脸猪、米猪、嘉兴黑猪、枫泾猪和沙乌头猪)和西方5个引进猪品种(杜洛克、长白猪、大白猪、皮特兰、巴克夏)进行简化基因组测序(genotyping by genome reducing and sequencing, GGRS),筛选出小梅山猪与其他猪种具有差异的5个SNP位点,采用Sanger测序进行验证。结果表明,上述5个SNPs在小梅山猪中存在较高等位基因突变率,利用5个候选位点的组合可达到鉴定概率99.35%、准确率100%,无假阳性错判。本研究为小梅山猪遗传资源保护、假冒伪劣品种辨别提供了参考依据。Small Meishan pig(Sus scrofa) is a unique local pig breed in the Taihu River Basin which has the characteristics of delicious meat. There is the problem of counterfeiting meat products in the market, while the traditional identification technology has some limitations in practical application. The present study is to develop a new method to distinguish Small Meishan pig from other pig breeds or strains of Taihu Basin and common western pig breeds by using the third generation molecular marker called Single Nucleotide Polymorphism(SNP). The data of genotyping by genome reducing and sequencing(GGRS) of 7 local breeds(strains) in Taihu Basin(Middle Meishan pig, Small Meishan pig, Erhualian pig, Mi pig, Jiaxinghei pig,Fengjing pig and Shawutou pig) and 5 imported pig breeds(Duroc, Landrace, Yorkshire, Piltland, and Baxia)was used. Five SNP sites only in Small Meishan pig were selected and Sanger sequencing was used to verify the results. It was showed that the 5 SNP sites had high mutation rate in Small Meishan pig. By using the combination of 5 candidate sites, the identification probability could be up to 99.35% and the accuracy was100% with no false positive identification. The present study provides a basic principle for the protection ofgenetic resources and identification of fake and inferior pork products in Small Meishan pig.

关 键 词：小梅山猪 品种鉴定 单核苷酸多态性(SNP) Sanger测序 

分 类 号：S813.23[农业科学—畜牧学]