温敏性荧光纳米微球的聚合物配方筛选与制备  

Polymer Screen and Preparation of the Thermo-Responsive Fluorescent Nanospheres

作　　者：盛扬 段宗权 孙一新 李坚 张嵘 Yang Sheng;Zongquan Duan;Yixin Sun;Jian Li;Rong Zhang(School of Materials Science and Engineering,Changzhou University;Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials;Jiangsu Collaboration Innovation Center of Photovoltaic Science and Engineering,Changzhou 213164,China)

机构地区：[1]常州大学材料科学与工程学院 [2]江苏省环境友好型高分子材料重点实验室 [3]江苏光伏科技合作创新中心,江苏常州213164

出　　处：《高分子材料科学与工程》2018年第11期137-143,150,共8页Polymer Materials Science & Engineering

基　　金：江苏省自然科学基金青年基金资助项目(BK20160278);常州大学科研启动基金(ZMF15020107);江苏省双创博士计划

摘　　要：文中使用喷墨打印的方法制备包埋有荧光共轭聚合物(FCP)的聚丙烯酸酯微点阵列芯片,通过对微点的荧光强度分析筛选出对FCP具有荧光增益效应的聚合物配方,并以此配方进行放大实验制备了包埋有荧光共轭聚合物的荧光纳米微球。通过红外光谱、紫外、扫描电子显微镜、透射电子显微镜、荧光分析等对荧光纳米微球的组成及性能进行了分析。结果表明,通过控制单体的配方比例可获得使FCP荧光强度增强的荧光纳米微球,并且发现其具有温敏性,荧光强度随着温度的升高而减弱,降低而恢复。细胞毒性及成像实验表明,荧光纳米微球具有良好的生物相容性,在细胞成像方面有良好的应用前景。In this work,acrylate polymer microarrays encapsulated with a fluorescent conjugated polymer (FCP) were prepared by inkjet printing approach followed with UV initiated in situ polymerization.The fluorescent intensity of each spot on the arrays was analyzed for the identification of the monomer compositions that enhanced the fluorescent intensity of the embedded FCP.The identified polymer candidates were then up-scaled by synthesizing fluorescent polymer nanospheres.The chemical composition and fluorescent properties were analyzed by FT-IR,UV- vis,SEM,TEM and fluorescence spectroscopy.The as-synthesized nanospheres show reversible fluorescent intensity change following switching of temperature,higher temperature makes fluorescent intensity weaker and the fluorescent intensity is recovered as temperature reducing.In vitro cell viability assay shows that the nanospheres loaded with FCP are cell compatible with low cytotoxicity.Therefore,they are promising to be used as fluorescent probes for cellular imaging and tracking.

关 键 词：聚合物微点阵列芯片 荧光共轭聚合物 荧光强度 生物相容性 细胞成像 

分 类 号：TB383[一般工业技术—材料科学与工程]