凤丹内生真菌Fusarium oxysporum次生代谢产物的研究  

Secondary metabolites from endophytic fungus Fusarium oxysporum isolated from Paeonia ostii

作　　者：王举涛 张培良 王刚 王国凯 刘劲松 许凤清 刘海涛 吴培云 WANG Ju-tao;ZHANG Pei-liang;WANG Gang;WANG Guo-kai;LIU Jin-song;XUFeng-qing;LIU Hai-tao;WU Pei-yun(Anhui Engineering Research Center of Modernized Chinese Material,School of Pharmacy,Anhui University of Chinege Medicine,Heifei 230012,China)

机构地区：[1]安徽中医药大学药学院,现代中药安徽省工程技术研究中心,安徽合肥230012

出　　处：《中草药》2018年第22期5247-5253,共7页Chinese Traditional and Herbal Drugs

基　　金：植物化学与西部植物资源持续利用国家重点实验室(P2017一KF03);安徽省高等学校自然科学基金重点项目(KJ2017A294,KJ2016JA853);安徽省中央引导地方科技发展专项(2017070702D173).

摘　　要：目的研究凤丹内生真菌Fusarium oxysporum的次生代谢产物。方法运用多种色谱法对菌株F. oxysporum发酵液的醋酸乙酯萃取部位进行分离纯化,采用波谱分析对化合物的结构进行鉴定。结果从菌株F. oxysporum发酵液的醋酸乙酯萃取部位中共分离得到23个化合物,分别鉴定为(2S)-脯氨酸(1)、(22E,24R)-7,22-二烯-3β,5α,6β-麦角三醇(2)、环-(S-脯氨酸-S-亮氨酸)(3)、环-(L-丙氨酸-L-脯氨酸)(4)、环-(缬氨酸-脯氨酸)(5)、环-(R-脯氨酸-S-苯丙氨酸)(6)、环-(D-顺-4-羟脯氨酸-L-苯丙氨酸)(7)、环-(L-反-4-羟基脯氨酸-L-苯丙氨酸)(8)、环-(谷氨酸-酪氨酸)(9)、环-(L-4-反-羟基脯氨酸-L-亮氨酸)(10)、环-(L-色氨酸-L-脯氨酸)(11)、环-(L-亮氨酸-甘氨酸)(12)、N-(3-(1H-吲哚-3-基)丙基)乙酰胺(13)、吲哚-3-乙酸(14)、2-哌啶酮(15)、2-吡咯烷酮(16)、胸腺嘧啶(17)、(5S)-5-((4-羟基苯基)甲基)-2,4-咪唑烷二酮(18)、(3S,6S)-3-(2-丁基)-6-(1-羟乙基)-2,5-哌嗪二酮(19)、环-(丙氨酸-亮氨酸)(20)、环-(S-脯氨酸-S-苯丙氨酸)(21)、环-(酪氨酸-脯氨酸)(22)、环-(L-苯丙氨酸-L-酪氨酸)(23)。结论除化合物2、6、14外,其余化合物均为首次从该菌株发酵液中分离得到。Objective To study the secondary metabolites of endophytic fungus Fusarium oxysporum form Paeonia ostii.Methods The fermentation liquor of the fungal strain F.oxysporum were isolated and purified using various chromatographic methods.The structures of the compounds were identified by spectrum analysis.Results Twenty-three compounds were isolated from the fungal and their structures were identified as (2S)-proline (1),(22E,24R)-ergosta-7,22-diene-3β,5ct,6f3-triol.(2),cyclo-(S-Pro-S-Leu)(3), cyclo-(L-Ala-L-Pro)(4),cyelo-(Val-Pro)(5),cyclo-(R-Pro-S-Phe)(6),cyclo-(D-cis-Hyp-L-Phe)(7),cyclo-(trans-4-hydroxy- L-Pro-L-Phe)(8),cyclo-(Gla-Tyr)(9),cyclo-(trans-4-hydroxy-L-Pro-L-Leu)(10),L,L-cyclo-(Trp-Pro)(11),cyclo-(L-Leu-Gly)(12), N-(3-(1H-indol-3-yl)propyl)acetamide (13),indole-3-acetic acid (14),2-piperidone (15),2-pyrrolidinone (16),thymidine(17), (5S)-5-[(4-hydroxyphenyl)methyl]-2,4-imidazolidinedione (18),(3S,6S)-3-(butan-2-yl)-6-(1-hydroxyethyl)piperazine-2,5-dione (19), cyclo-(Ala-Leu)(20),eyclo-(S-Pro-S-Phe)(21),cyclo-(Tyr-Pro)(22),and cyclo-(L-Phe-L-Tyr)(23).Conclusion With the exception of compounds 2,6,and 14,the other compounds are isolated from the fermentation liquor of the fungal strain F. oxysporum for the f'trst time.

关 键 词：凤丹 内生真菌 FUSARIUM OXYSPORUM Schlecht. 次生代谢产物 (2S)一脯氨酸 环一降脯氨酸-S-苯丙氨酸) 

分 类 号：R284.1[医药卫生—中药学]