基于高效液相色谱-电感耦合等离子体质谱法的大鼠口服纳米雄黄后体内砷形态分析  

Determination of arsenic species in serum, liver, kidney, and spleen of realgar nanoparticles treated rats by HPLC-ICP-MS

作　　者：姜新[1] 李少元[2] 邓小颖 张尊建[2] 黄寅[2] JIANG Xin;LI Shao-yuan;DENG Xiao-ying;ZHANG Zun-jian;HUANG Yin(Physical and Chemical Analysis Department,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China;Key Laboratory of Drug Quality Control and Pharmacovigilance,Ministry of Education,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]江苏省疾病预防控制中心理化检验所,江苏南京210009 [2]中国药科大学药物质量与安全预警教育部重点实验室,江苏南京210009

出　　处：《中草药》2018年第22期5328-5333,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81403181);江苏省自然科学基金项目(BK20140664)

摘　　要：目的对大鼠口服雄黄与纳米雄黄后血清、肝、肾、脾中三价砷[As(III)]、五价砷[As(V)]、一甲基砷酸(MMA)、二甲基砷酸(DMA)4种砷形态进行定量分析,比较两组大鼠体内砷形态的差异。方法 SD大鼠分别ig给予800 mg/kg的雄黄与纳米雄黄,28 d后采集血清及各组织样本,经前处理后采用高效液相色谱-电感耦合等离子体质谱法(HPLC-ICP-MS)测定生物样本中砷形态含量并进行比较。结果 4种砷形态在大鼠血清和肾脏样本中均被检出,肝脏中检出了3种,脾脏中检出了2种;各砷形态在纳米雄黄组中的含量明显高于雄黄组。结论纳米化提高了雄黄的生物利用度,更多的砷被吸收进入体内并发生代谢转化,这可能导致纳米雄黄肝、肾等毒性增加。Objective Quantitative analysis of four arsenic species As (III),As (V),monomethyl arsenate (MMA),dimethyl arsenate (DMA)in rat serum,liver,kidney,and spleen was performed to compare their differences between realgar and realgar nanoparticles (NPs)groups.Methods SD rats were ig treated with blank solvent,realgar,and realgar NPs (800mg/kg)respectively.After 28d of continuous administration,serum and tissues were collected and four arsenic species were determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS).Results Four arsenic species were detected in serum and kidney of rats,three were detected in the liver and two in the spleen.The content of arsenic species in the realgar NPs group was significantly higher than that in the realgar group.Conclusion Nanotechnology enhanced the bioa;eailability of realgar,and more arsenic was absorbed into the body and underwent metabolic transformation,which might lead to increased toxicity ofrealgar NPs.

关 键 词：纳米雄黄 砷形态 高效液相色谱-电感耦合等离子体质谱法 砷毒性 三价砷、五价砷、一甲基砷酸、二甲基砷酸 

分 类 号：R285.5[医药卫生—中药学]