野生与栽培远志HPLC指纹图谱及化学模式识别研究  

HPLC fingerprint analysis and chemistry pattern recognition of wild and cultivated Polygalae Radix from different regions

作　　者：彭亮[1] 杨冰月[1] 张琳[1] 周可心 颜永刚[1] 李依民[1] 高静[1] 陈莹[1] 王薇[1] 张岗[1] 胡本祥[1] PENG Liang;YANG Bing-yue;ZHANG Lin;ZHOU Ke-xin;YAN Yong-gang;LI Yi-min;GAO Jing;CHEN Ying;WANG Wei;ZHANG Gang;HU Ben-xiang(College of Pharmacy,Shaanxi University of Chinese Medicine Xi'an 712046,China)

机构地区：[1]陕西中医药大学药学院,陕西西安712046

出　　处：《中草药》2018年第21期4998-5003,共6页Chinese Traditional and Herbal Drugs

基　　金：公益性行业(中医药)科研专项经费项目(201507002);国家大学生创新计划(201710716011);陕西省高校青年杰出人才支持计划项目;咸阳市中青年科技领军人才项目

摘　　要：目的建立野生和栽培远志的HPLC指纹图谱,为不同来源远志鉴别和质量评价提供依据。方法利用HPLC法对20批不同产地野生与栽培远志进行测定,采用中药色谱指纹图谱相似度评价系统(2012A版)计算相似度,运用主成分分析(PCA)和最小偏二乘法-判别分析(PLS-DA)对其进行模式识别研究。结果建立了野生与栽培远志HPLC指纹图谱共有模式,标定了24个共有峰,20批不同产地野生与栽培远志指纹图谱与对照指纹图谱的相似度均>0.86。PCA可较好地将不同来源远志归为2类,PLS-DA则能将野生与栽培远志完全区分开,远志酮III和3,6′-二芥子酰基蔗糖等12个成分是导致二者差异的主要标记物。结论建立的远志指纹图谱特征性强,结合化学模式识别方法可有效评价远志质量以及区分其野生与栽培品,为远志的质量控制和化学计量分类学提供参考依据。Objective To study and establish the HPLC fingerprint of Polgala tenuifolia for the identification and quality controlof P.tenuifolia.Methods Twenty batches of wild and cultivated P.tenuifolia collected from different regions in China were detected by HPLC.The Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 A edition) was used to evaluate the similarity of the samples.The differences among the samples were identified by chemical pattern recognition methods including principal component analysis(PCA) and partial least squares discriminate analysis(PLS-DA).Results The common model of HPLC fingerprint of wild and cultivated P.tenuifolia was obtained,24 common peaks were found in the chromatograph.The similarities between wild and cultivated P.tenuifolia fingerprints and control fingerprints in 20 batches from different regions were over 0.86,PCA results demonstrated obvious distinction between the wild and cultivated P.tenuifolia.The wild and cultivated P.tenuifolia was completely distinguished by PLS-DA.Twelve constituents,such as polygalactoneone III and 3,6′-disinapoyl sucrose were screened as biomarkers,representing the major differences between the two varieties.Conclusion The HPLC fingerprint combined with chemical pattern recognition can be used as an effective method for the quality control and identification of the different sources of P.tenuifolia,and provide a reference for the quality control and stoichiometric taxonomy of P.tenuifolia.

关 键 词：远志 HPLC 指纹图谱 化学模式识别 远志[口山]酮Ⅲ 3 6’-二芥子酰基蔗糖 主成分分析 

分 类 号：R286.2[医药卫生—中药学]