新生期肺炎链球菌肺炎对气道平滑肌表型的影响  

Effects of neonatal pneumococcal pneumonia on airway smooth muscles

作　　者：彭欣 吴毅[1] 陈云秀[1] 田永露 李媛媛[1] 张光莉[2] 田小银 罗征秀[2] 李沁原 PENG Xin;WU Yi;CHEN Yunxiu;TIAN Yonglu;LI Yuanyuan;ZHANG Guangli;TIAN Xiaoyin;LUO Zhengxiu(Ministry of Education Key Laboratory Chongqing 400014,China;Department University,Chongqing 400014,China of Child Development and Disorder,of Respiratory Medicine,Children's Chongqing Medical University,Hospital of Chongqing Medical)

机构地区：[1]儿童发育疾病研究省部共建教育部重点实验室、儿科学重庆市重点实验室、重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]重庆医科大学附属儿童医院呼吸科,400014

出　　处：《免疫学杂志》2018年第12期1034-1040,共7页Immunological Journal

基　　金：国家自然科学基金(81270086);重庆市科委重点项目(cstc2017jcyjB0160);重庆市渝中区科委项目(20170120).

摘　　要：目的研究新生期肺炎链球菌肺炎(Streptococcus pneumoniae pneumonia,S.pp)对气道平滑肌表型的影响。方法Balb/c小鼠出生后第7天鼻腔滴注2×107CFU肺炎链球菌5μl建立新生期S.pp模型,对照组滴注等量PBS。感染5周后收集肺组织标本,免疫组织化学检测气道平滑肌肌层厚度;RT-PCR检测气道平滑肌Acta2-mRNA、My11-mRNA、Tagln-mRNA表达量;Western blot检测α-SMA、SMMHC、SM22α蛋白表达量。结果 S.pp组气道平滑肌面积(α-SMA+area/Pbm)较对照组显著增加(46.06±10.74 vs 22.50±9.131,P<0.01),平滑肌收缩蛋白SMMHC面积(SMMHC+area/Pbm)较对照组明显增加(58.33±29.40 vs19.75±13.10,P<0.05),2组间平滑肌收缩蛋白SM22α面积(SM22α+area/Pbm)无统计学差异(37.20±10.67 vs 41.33±5.871,P>0.05);Western blot分析发现S.pp组小鼠肺组织α-SMA、SMMHC蛋白较对照组显著升高(分别为1.352±0.552 9 vs 0.633 0±0.157 5,P<0.05;1.291±0.487 2 vs 0.776 8±0.279 3,P<0.01),肺组织SM22α蛋白表达水平差异无统计学意义(1.435±0.436 5 vs 1.398±0.437 3,P>0.05);RT-PCR检测发现S.pp组Acta2-mRNA、My11-mRNA表达水平较对照组显著增加(分别为2.704±1.044 vs0.906 5±0.214 8,P<0.01;1.780±0.454 6 vs 1.183±0.369 9,P<0.05),而2组间Tagln-mRNA表达水平无统计学差异(1.438±0.321 3vs 1.207±0.334 5,P>0.05)。结论新生期肺炎链球菌肺炎可诱导气道平滑肌表型改变,促进气道高反应性形成。To investigate the effect of neonatal Streptococcus pneumoniae pneumonia (S.pp)on airway smooth muscle phenotypes,neonatal Balb/c mice (1-week-old)were infected intranasally with 2×10^7 CFU of S.pneumoniae (D39)in 5μl of PBS (S.pp group)or with 5μl of PBS (control group).Five weeks after infection,the lung tissue was collected for evaluating airway smooth muscles and their contractile proteins by immunohistochemical staining,RT - PCR and Western blotting.Data showed that airway smooth muscle area (α-SMA^+ area/Pbm)and airway smooth muscle myosin heavy chain area (SMMHC^+ area/Pbm)were significantly increased in S.pp group (46.06±10.74 vs 22.50±9.131,P<0.01;58.33±29.40vs 19.75±13.10,P< 0.05)compared with control group,while airway smooth muscle 22 alpha area (SM22 α^+area/Pbm)was similar between S.pp and control group (37.20±10.67 vs 41.33±5.871,P>0.05).As expected,α-SMA and SMMHC proteins were significantly higher in S.pp group than those in control mice (1.352±0.5529 vs 0.6330±0.1575,P<0.05;1.291±0.4872 vs 0.7768± 0.2793,P<0.01,respectively).SM22 α protein was similar between the two groups (1.435±0.4365 vs 1.398±0.4373, P>0.05).Meanwhile,Acta 2-mRNA and Myh11-mRNA were significantly higher in S.pp group than those in control mice (2.704±1.044 vs 0.9065±0.2148,P<0.01;1.780±0.4546 vs 1.183±0.3699,P<0.05,respectively);Tagln-mRNA was similar between the two groups (1.438±0.3213 vs 1.207±0.3345,P>0.05).In conclusion,neonatal Streptococcus pneumoniae pneumonia alters airway smooth muscles phenotypes and promotes airway hyperresponsiveness.

关 键 词：肺炎链球菌肺炎 新生期 气道平滑肌 气道高反应性 

分 类 号：R392[医药卫生—免疫学] R378.1[医药卫生—基础医学]