铁代谢异常对贫血患者EPO-STAT5信号通路的影响  

Effects of Abnormal Iron Metabolism on EPO-STAT5 Signaling Pathway in Anemia Patients

作　　者：张耀[1] 韩爽[1] 郭垞[1] 张青霞[1] 常春康[1] ZHANG Yao;HAN Shuang;GUO Cha;ZHANG Qing-Xia;CHANG Chun-Kang(Department of Hematology,East Hospital of The Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 201306,China)

机构地区：[1]上海交通大学附属第六人民医院东院血液科,上海201306

出　　处：《中国实验血液学杂志》2018年第6期1726-1730,共5页Journal of Experimental Hematology

摘　　要：目的:研究铁代谢异常对贫血患者EPO-STAT5信号通路的影响。方法:根据疾病将患者分为较低危骨髓增生异常综合症(MDS)组30例,其中铁过载16例,非铁过载14例,对其中12例铁过载患者进行祛铁治疗;慢性病贫血(ACD)组(12例),缺铁性贫血(IDA)组(12例),另设健康对照组10例;检测患者铁代谢指标铁蛋白(SF),血清铁(SI)、总铁结合力(TIBC),血清红细胞生成素(EPO)、血浆中P-STAT5及外周血中STAT5 mRNA表达。分析铁代谢异常对各组贫血患者EPO及STAT5表达的影响。结果:与非铁过载组相比,铁过载组MDS患者EPO水平明显升高(P <0.05),STAT5 mRNA及P-STAT5表达明显降低(P <0.05)。祛铁治疗后EPO水平明显下降(P <0.05),STAT5 mRNA及P-STAT5表达明显升高(P <0.05)。与健康对照组相比,ACD组EPO表达明显下降(P <0.05),STAT5 mRNA表达无差异,P-STAT5表达明显降低(P <0.05);IDA组EPO表达明显升高(P <0.05),STAT5 mRNA及P-STAT5表达明显升高(P <0.05)。结论:过量铁负荷或慢性炎症可能抑制EPO-STAT5通路激活,加重贫血。Objective: To study the effects of iron metabolism abnormality on EPO-STAT5 signaling pathway in anemia patients.Methods: According to diseases, the patients were divided into 3 groups: lower risk myelodysplastic syndrome (MDS) group (30 cases) including 14 cases of non-iron over load and 16 cases of iron over load, 12 cases of them were treated by iron chelation therapy; anemia of chronic disease (ACD) group (12 cases) and iron deficiency anemia (IDA) group (12 cases).In addition, the healthy control group was selected.The iron metaloslism index (SF, SI, TIBC), serum level of EPO, plasma level of P-STAT5 and STAT-5 mRNA expression in peripheral blood cells were detected and compared in different groups.Moreover, the effects of iron metabolism abnormality on the expression of EPO and STAT5 in anemia patients were analyzed.Results: compared with non-iron over load group, the EPO level in iron over load group significantly increased (P<0.05), the expression of STAT5 mRNA and P-STAT5 significantly decreased (P<0.05).After iron chelation therapy, the EPO level in serum significantly decreased (P<0.05), the expression of STAT5 mRNA and P-STAT5 was up-regulated significantly (P<0.05).Compared with healthy control group, the expression of EPO in ACD group was down-regulated significantly, while the expression of STAT5 mRNA was not different, but the P-STAT5 expression was down-regulated significantly (P<0.05).Compared with the healtly control group, the EPO expression in IDA group was enhanced significantly (P<0.05), the expression of STAT5 mRNA and P-STAT5 were also significantly enhanced (P<0.05).Conclusion: The excessive iron load or chronic inflammation may inhibit the activation of EPO-STAT5 signaling pathway and aggravate the anemia.

关 键 词：贫血 铁代谢 EPO—STAT5信号通路 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]