CD180在B细胞慢性淋巴增殖性疾病中的表达特点及其诊断价值  

Characteristics of CD180 Expression and Its Diagnostic Value in B Cell Chronic Lymphoproliferative Disorders

作　　者：吴婧[1] 盛燕[1] 眭竫旎 翁香琴[1] WU Jing;SHENG Yan;SUI Jing-Ni;WENG Xiang-Qin(Shanghai Institute of Hematology,Ruijin Hospital Affiliated Shanghai Jiaotong University School of Medicine,Shanghai,200025,China)

机构地区：[1]上海交通大学医学院附属瑞金医院血液学研究所,上海200025

出　　处：《中国实验血液学杂志》2018年第6期1811-1815,共5页Journal of Experimental Hematology

摘　　要：目的:通过多参数流式细胞术(flow cytometry,FCM)检测B细胞慢性淋巴增殖性疾病(B cell chronic lymphoproliferative disorders,B-CLPD)中CD180的平均荧光强度(mean fluorescence intensity,MFI),探讨CD180在B-CLPD中的表达特点及其在鉴别诊断中的价值。方法:利用FCM检测178例B-CLPD病人骨髓中异常B淋巴细胞表面CD180的MFI。以正常对照组为参照,分析各类型B-CLPD中CD180的表达情况,并比较不同亚型中的表达差异。结果:(1)除脾脏弥漫性红髓小B细胞淋巴瘤(SDRPL)外,各类型B-CLPD中CD180的表达强度均显著低于正常对照组;(2)慢性淋巴细胞性白血病(chronic lymphocytic leukemia,CLL)中CD180 MFI明显低于其余类型B-CLPD;(3) CD180在毛细胞白血病(HCL)中的表达强度显著高于边缘区淋巴瘤(MZL)、套细胞淋巴瘤(MCL)、淋巴浆细胞淋巴瘤/华氏巨球蛋白血症(LPL/WM);(4)在脾脏边缘区淋巴瘤(SMZL)、毛细胞白血病(HCL)、毛细胞白血病变异型(HCLv)以及SDRPL等原发于脾脏的淋巴瘤中,有绒毛组和无绒毛组CD180表达强度有显著差异。结论:利用多参数FCM,检测CD180和其他免疫标志表达水平,有助于区分B-CLPD不同亚型。Objective: To investigate the charactcristics of CD180 expression and differentiation diagnostic value in B cell chronic lymphoproliferative disorders (B-CLPD) through detecting the mean fluorescence intensity(MFI)of CD180 in different sub types of B-CLPD,using multiparameter flow cytometry (FCM).Methods: The CD180 MFI of malignant B cells in 178 patients with B-CLPD was detected by FCM.The level of CD180 MFI in various types of B-CLPD was compared to the normal control group.The level of CD180 MFI among sub-types of B-CLPD was also compared.Results:(1) The expression levels of CD180 in B-CLPD was significantly lower as compared with the normal controls,except the spleen difuse red pulp lymphoma (SDRPL); (2) The CD180 MFI in chronic lymphocytic leukemia sCLL) was significantly lower as compared with other B-CLPD cells; (3) CD180 ware significantly overexpressed in HCL compared with MCL,LPL,and MZL (P<0.05); (4) In the spleen-derived B-CLPD,such as SMZL,HCL, HCL variation and SDRPL,the expression of CD180 has significant difference between lymphomas with or without villous.Conclusion: Utilizing the multiparameter flow cytometry for defecting expression of CD180 and other immunological markers can more efficiently distinguish the subtypes of B-CLPD.

关 键 词：B细胞慢性淋巴增殖性疾病 CD180平均荧光强度 多参数流式细胞术 

分 类 号：R551.3[医药卫生—血液循环系统疾病]