儿童肛门生殖器疣45例HPV病毒检测分析  

HPV Genotype Detection in 45 Cases of Children with Ano-genital Warts

作　　者：黄丹[1] 张小华[1] 朱艳 顾恒[1] 鞠梅[1] 陈崑[1] HUANG Dan;ZHANG Xiaohua;ZHU Yan;GU Heng;JU Mei;CHEN Kun(Jiangsu Key Laboratory of Molecular Biology for Skirt Diseases and STIs ,Institute of Dermatology,Chinese Academy of Medical Sciences and Peking Union Medical College,Nanjing 210042,China)

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所江苏省皮肤病与性病分子生物学重点实验室,江苏南京210042

出　　处：《中国皮肤性病学杂志》2018年第12期1407-1410,共4页The Chinese Journal of Dermatovenereology

基　　金：江苏省自然科学基金(BK20160152);中国医学科学院医学与健康科技创新工程重大协同创新项目(CIFMS-2017-I2M-1-017)

摘　　要：目的检测儿童肛门生殖器疣的HPV病毒基因型。方法门诊收集12岁以下的儿童肛门生殖器疣患者,记录病史及查体,留取皮损处脱落细胞标本,运用PCR-反向点杂交法检测15种高危型别,3种疑似高危型别,10种低危型别和1种皮肤型别。统计患者的一般情况、皮损分布和HPV检查结果。结果共收集了45例4个月～12岁的儿童肛门生殖器疣患者,男女分别为12例,23例,男女比例0. 4∶1,年龄中位值4岁,病程4个月。皮损分布在肛周者最多为38例(84. 44%),其次外生殖器8例(17. 78%)。8例(17. 78%)为多部位发病,其余37例(82. 22%)均为单部位发病,单发于肛周者最多为30例(66. 67%)。HPV检测阳性者有24例,阳性率53. 33%,6型感染16例(66. 67%),11型感染4例(16. 67%),7型感染4例(16. 67%),59+6型混合感染1例。HPV6型在性别间分布差异有统计学意义(P=0. 023)。结论本地区儿童肛门生殖器疣HPV感染亚型以低危型6型为主,多为单一型感染,皮肤型感染的比率可能被低估。Objective To detect the HPV genotypes in children with anal and genital warts.Methods Investigated the medical history,took physical examination in patients under 12 years with ano-genital warts then collected the exfoliated cells on lesions.PCR-reverse dot blot method was adopted to detect the HPV genotype including 15 high-risk viruses,3 suspected high-risk virus,10 low-risk virus and 1 cutaneous type.The general condition was described,skin lesion distribution and HPV test were analyzed between genders. Results A total of 45 child were enrolled,the average age and process of disease [M( P25 ~ P75) ] were 4( 2 ~ 6) y,4( 2 ~ 12) m,separately.Sex ratio was 0. 4∶ 1( 12 boys and 23 girls). 38 cases( 84. 44%) of them were suffered from perianal,followed by genital 17. 78%. 8 cases( 17. 78%) had multiple sites involed,the rest( 82. 22%) are single site,especially anal( 66. 67%). There was no statistical difference between genders on lesion distribution. HPV positive test for 24 cases,the positive rate was 53. 33%. The most common type was HPV6,fol-lowed by HPV11,HPV7,and HPV59 + HPV6. The virus type has no statistically significant differences between genders except HPV6. Conclusion HPV6 or 11 were the main genotype to cause Child ano-genital warts,additionally,the ratio of cutaneous type infection may be underestimated.

关 键 词：疣 生殖器 儿童 人乳头瘤病毒 基因型 

分 类 号：R752.5[医药卫生—皮肤病学与性病学]