补益宗气方对慢性阻塞性肺疾病大鼠营养状态、血清TNF-α及MSTN水平的影响  

Influence of Tonifying Zongqi Formula in chronic obstructive pulmonary disease rats about the nutritional status and the level of serum prealbumin, leptin, TNF-α and myostatin

作　　者：兰智慧[1] 陈章生[1] 宋文龙 黄春燕 刘良徛[1] LAN Zhi-hui;CHEN Zhang-sheng;SONG Wen-long;HUANG Chun-yan;LIU Liang-ji(Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;Jiande Hospital of Integrated Chinese and Western Medicine,Jiande 311612,China;Jiangxi University of Traditional Chinese Medicin,Nanchang 330006,China)

机构地区：[1]江西中医药大学附属医院,南昌330006 [2]浙江省建德市中西医结合医院,浙江建德311612 [3]江西中医药大学,南昌330006

出　　处：《中华中医药杂志》2018年第12期5381-5385,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81760859;No.81560765);江西省自然科学基金项目(No.20171BAB205081);江西省卫生计生委中医药科研课题(No.2014A010)~~

摘　　要：目的:观察补益宗气方对COPD大鼠营养状态及血清肿瘤坏死因子(TNF-α)、肌抑素(MSTN)水平的影响,探讨其改善COPD营养不良的可能作用机制。方法:将30只SD大鼠,随机分为空白组、模型组、治疗组,以气管内注射脂多糖加烟雾吸入及冷风刺激建造COPD模型大鼠,治疗组给予补益宗气方灌胃,采用酶联免疫吸附方法对各组大鼠血清前白蛋白、瘦素、TNF-α、MSTN水平进行检测。结果:(1)补益宗气方干预前,模型组、治疗组血清TNF-α及MSTN水平均明显高于空白组(P<0.05),血清前白蛋白及瘦素水平明显低于空白组(P<0.05);补益宗气方干预后,治疗组血清中TNF-α及MSTN含量比模型组显著降低(P<0.05),血清前白蛋白和瘦素水平则明显高于模型组(P<0.05);(2)补益宗气方干预后,治疗组体质量和Lee’s指数较模型组上升明显(P<0.05)。结论:补益宗气方能增加COPD大鼠体质量和Lee’s指数,改善营养状态,其作用机制可能与其降低TNF-α及MSTN水平,提高前白蛋白和瘦素水平有关。Objective: To observe the effect of Tonifying Zongqi Formula on nutritional status the level of serum TNF-αand myostatin in rats with chronic obstructive pulmonary disease(COPD) and to discuss the possible mechanism. Methods: SD rats were randomized into 3 groups: blank group, model group, treatment group. Use the smoked cigarettes, cold air stimulation plus endotracheal lipopolysaccharide injection to establish the rat model with COPD and treatment group were treated with Tonifying Zongqi Formula, and enzyme-linked immunosorbent assay(ELISA) was carried to detecte the levels of serum prealbumin, leptin, TNF-α and myostatin of all rats. Results:(1)Before the Intervention of Tonifying Zongqi Formula, the level of TNF-α and myostatin of model group and treatment group were much higher than blank group(P<0.05), while the level of serum prealbumin and leptin was significantly lower than that of control(P<0.05). After the Intervention the level of TNF-α and Myostatin of treatment group were lower than model group(P<0.05). While the level of Serum prealbumin and leptin was significantly higher than that of model group(P<0.05).(2)Compared with the model group, the body weight and Lee’s index of the treatment group after the Intervention of Tonifying Zongqi Formula increased significantly(P<0.05). Conclusion: Tonifying Zongqi Formula can increase body weight of rats with COPD and improve the nutritional status, and its mechanism of action may related to the reduction of TNF-α and the level of myostatin and the improvement of the level of prealbumin and leptin.

关 键 词：慢性阻塞胜肺疾病 营养不良 补益宗气 肿瘤坏死因子 肌抑素 

分 类 号：R285.5[医药卫生—中药学]