肾阳虚股骨颈骨折患者松质骨组织差异蛋白质的表达分析  

Analysis of differential protein expression of cancellous bone tissue in femoral neck fracture patients with syndrome of deficiency of kidney yang

作　　者：翁绳健[1] 吴立忠[1] 李炜明[1] 詹洋[1] 吴星[1] 康荣彬 郑萍[1] 郭洁梅[2,3] 滕方舟 王建辉 朱亚菊 李保林 苏友新 WENG Sheng-jian;WU Li-zhong;LI Wei-ming;ZHAN Yang;WU Xing;KANG Rong-bin;ZHENG Ping;GUO Jie-mei;TENG Fang-zhou;WANG Jian-hui;ZHU Ya-ju;LI Bao-lin;SU You-xin(Fuzhou Second Hospital,Fuzhou 350007,China;Fujian Health College,Fuzhou 350101,China;Fujian University of Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福州市第二医院,福州350007 [2]福建卫生职业技术学院,福州350101 [3]福建中医药大学,福州350122

出　　处：《中华中医药杂志》2018年第12期5397-5401,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省自然科学基金项目(No.2015J01360).

摘　　要：目的:比较并分析肾阳虚与非肾虚患者股骨颈松质骨差异表达的蛋白质,从骨组织功能蛋白质角度初步探讨"肾主骨"理论科学内涵。方法:收集肾阳虚患者及非肾虚患者的股骨颈松质骨标本各13例,进行骨总蛋白提取、双向电泳(2-DE)蛋白质分离、凝胶图谱的获取、质谱分析及生物信息学检索。结果:2-DE获得的肾阳虚及非肾虚患者股骨颈松质骨可辨识总蛋白点数目为582个,与非肾虚组比较,肾阳虚证组有16个蛋白出现差异表达。其中线粒体热休克蛋白90(Hsp90s)等5个蛋白表达上调;骨形态发生蛋白4(BMP-4)等11个蛋白表达下调。结论:股骨颈松质骨16个蛋白表达改变可能与肾阳虚证的发生有关,"肾"可能通过调节这些与骨组织关系密切的蛋白表达而发挥"主骨"作用。Objective:To compare and analyze the differential expression of the protein in cancellous bone of femoral neck in patients with the syndrome of deficiency of kidney yang and non-deficiency of kidney yang,and to explore the scientific connotation of the theory of ‘kidney dominating bone'from the angle of bone tissue function protein.Methods:Thirteen specimens of cancellous bone of femoral neck were collected from patients with the syndrome of deficiency of kidney yang and 13 patients with non-deficiency of kidney yang.We extracted total protein from patients'cancellous bone of femoral neck and made an analysis of two-dimensional electrophoresis (2-DE)and gel chromatogram and a bioinformatics analysis of related functions of some differentially expressed protein spots.Results:A total of 582 protein spots were identified in cancellous bone of femoral neck of patients with the syndrome of deficiency of kidney yang and non-deficiency of kidney yang.Compared with the non-deficiency of kidney yang group,16 proteins were differentially expressed in the deficiency of kidney yang group.Among them,mitochondrial heat shock protein 90 (Hsp90s)and other 5 proteins were upregulated,and bone morphogenetic protein 4 (BMP-4)and other 11 proteins were downregulated.Conclusion:The expression changes of 15 proteins in cancellous bone of femoral neck may be related to the occurrence of the syndrome of deficiency of kidney yang,and the kidney may play a role of ‘dominating bone'by regulating the expression of these proteins which are closely related to bone tissue.

关 键 词：差异蛋白质 肾阳虚证 股骨颈松质骨 双向电泳 质谱 

分 类 号：R274.1[医药卫生—中医骨伤科学]