芩楼清利丸对变态反应性接触性皮炎大鼠血清及皮损炎性因子的影响  

Effects of Qinlou Qingli Pills on serum and inflammation of skin lesion part of allergic contact dermatitis model of rats

作　　者：梅沉成 何文凤 李瑞婷 孙超逸[4] 范欢[4] 任映[5] 王蓬文[5] 屈双擎[4] 张云璧[4] MEI Chen-cheng;HE Wen-feng;LI Rui-ting;SUN Chao-yi;FAN Huan;REN Ying;WANG Peng-wen;QU Shuang-qing;ZHANG Yun-bi(Beijing University of Chinese Medicine,Beijing 100029,China;Department of Dermatology,Fangshan Traditional Medical Hospital of Beijing,Beijing 102488,China;Department of Dermatology,Zhengzhou Traditional Chinese Medicine Hospital,Zhengzhou 450000,China;Department of Dermatology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Key Laboratory of Chinese Internal Medicine,Ministry of Education,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京市房山区中医院皮肤科,北京102488 [3]郑州市中医院皮肤科,郑州450000 [4]北京中医药大学东直门医院皮肤科,北京100700 [5]北京中医药大学东直门医院中医内科学教育部重点实验室,北京100700

出　　处：《中华中医药杂志》2018年第12期5592-5595,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中央高校基本科研业务费专项资金(No.2018-JYBZZ-XS033);北京市中医药科技项目(No.JJ2014-57)~~

摘　　要：目的:探讨芩楼清利丸治疗变态反应性接触性皮炎(ACD)的可能作用机制。方法:将40只雄性SD大鼠随机分为空白组、模型组、治疗组和阳性对照组,各10只。除空白组外,其余3组采用0.1%二硝基氯苯溶液(DNCB)分别致敏2次建立ACD模型。治疗组将芩楼清利丸溶于纯净水中灌胃给药,阳性对照组将盐酸西替利嗪片溶于纯净水中灌胃给药,模型组采用纯净水灌胃,每天2次,共2周。实验2周后取材前评估皮损疗效;取材后,通过HE染色观察各组大鼠皮肤的病理变化;采用ELISA检测各大鼠血清中白细胞介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)以及皮损组织中白细胞三稀B4(LTB4)水平;采用实时荧光定量PCR检测皮损中白细胞三烯受体1(BLT1)、半胱氨酸天冬氨酸蛋白酶-14(Caspase-14)表达情况。结果:治疗组、阳性对照组与模型组相比,皮损红斑、水肿、丘疹、表皮剥脱等均显著减轻(P<0.05)。病理结果提示,两组大鼠背部皮肤的角质层变薄,表皮层、真皮层内炎症细胞浸润较模型组显著减少。模型组LTB4、TNF-α、Caspase-14 mRNA的表达水平较空白组显著上升(P<0.01,P<0.05),治疗组和阳性对照组LTB4较模型组显著下降(P<0.05,P<0.01),TNF-α、Caspase-14 mRNA的表达水平较模型组也显著下降(P<0.01,P<0.05)。模型组大鼠皮肤BLT1 mRNA的表达水平较空白组显著降低(P<0.01),阳性对照组和治疗组的表达水平较模型组上升,但差异无统计学意义。各组大鼠血清中IL-4水平无明显差异。结论:芩楼清利丸治疗ACD的作用机制可能与调节血清及皮损中的相关炎性因子及促进表皮屏障恢复有关。Objective:To explore the mechanism of Qinlou Qingli Pills on the allergic contact dermatitis (ACD). Methods:Forty male SD 102488 rats were randomly divided into blank group,model group,treatment group and positive control group.The ACD model was established by 0.1% of DNCB solution in twice hair removal department,and then treated with medicine after the second stimulation.Evaluating skin efficacy and observing the pathological changes by HE staining. The concentration of IL-4,TNF-α and LTB4 in serum were detected by ELISA.The mRNA expression of BLT1,Caspase-14 in skin lesion were tested by Real Time-Polymerase Chain Reaction (RT-PCR).Results:Compared with the model group,the skin damage was reduced significantly in the treatment group and positive control group (P<0.05).HE staining show that:the skin of the rats in the model group was mild hyperkeratosis,and a large number of inflammatory cells infiltrated in the epidermis and dermis.While in positive control group and the treatment group,the stratum eorneum became thinner,and the infiltration of inflammatory cells in epidermis and dermis was significantly lower than that in the model group.The levels of LTB4, TNF-α and Caspase-14 mRNA in model group were significantly higher than that of the blank group (P<0.01,P<0.05),while in the positive control group and treatment group the levels of LTB4 decreased significantly than that of model group (P<0.05, P<0.01),TNF-α and Caspase-14 mRNA also decreased significantly than that of model group (P<0.01,P<0.05),The expression of BLT1 mRNA in the skin lesion of model group were significantly lower than that of the blank group (P<0.01).The serum levels of IL-4in each groups had no significant difference.Conclusion:Qinlou Qingli Pills may reduce the inflammatory response and could be beneficial to the skin barrier function,which may be the mechanisms of of treating allergic dermatitis.

关 键 词：芩楼清利丸 变态反应性接触性皮炎 白细胞三稀B4 肿瘤坏死因子-Α 半胱氨酸天冬氨酸蛋白酶-14 炎性因子 

分 类 号：R285.5[医药卫生—中药学]