院内鲍曼不动杆菌同源基因型与耐药分析探讨  

An Analysis of Homologous Genotype and Drug Resistance of Acinetobacter Baumannii in Hospital

作　　者：郭晶晶[1] 王慧珠[1] 滑明溪 何明 孙继云[1] 张淑凤 王雅杰 GUO Jing-jing;WANG Hui-zhu;HUA Ming-xi;HE Ming;SUN Ji-yun;ZHANG Shu-feng;WANG Ya-jie(Department of Clinical Laboratory,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China;Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing Key Laboratory of Emerging Infectious Diseases,Beijing 100015,China;Department of Hospital Infection Management,Beijing Ditan Hospital,Capital Medical University ,Beijing100015,China)

机构地区：[1]首都医科大学附属北京地坛医院检验科,北京100015 [2]首都医科大学附属北京地坛医院研究所,北京100015 [3]首都医科大学附属北京地坛医院感染管理处,北京100015

出　　处：《标记免疫分析与临床》2018年第11期1589-1594,共6页Labeled Immunoassays and Clinical Medicine

基　　金：首都医科大学附属北京地坛医院重点实验室开放课题(编号:DTKF201702)

摘　　要：目的了解我院鲍曼不动杆菌的分子流行特征,分析其基因同源性,为医院感染控制提供实验室依据,探讨多位点序列分型技术的应用。方法收集本院分离的50株鲍曼不动杆菌,采用多位点序列分型(multilocus sequence typing,MLST)技术,PCR(Polymerase Chain Reaction)扩增gltA、gyrB、gdhB、recA、cpn60、gpi和rpoD七个管家基因片段,测序结果与PubMLST数据库对比分析,利用Phyloviz-2. 0软件绘制不同基因型之间的进化关系图。结果 50株鲍曼不动杆菌可被分为10个基因型,分别是ST-1696(19株)、ST-1144(14株)、ST-229(4株)、ST-191(4株)和ST-540、ST-1680、ST-208、ST-1679、ST-1145、ST-1421(分别各一株),三个新的基因型(3株),其中ST-1696与ST-1144、ST-229、ST-191、ST-540、ST-1680、ST-208、ST-1679、ST-1145、ST-1421基因型具有同源相关性。已有ST型的47株鲍曼不动杆菌中大多数菌株对临床常用抗生素表现出多重耐药性,抗生素的耐药率均在75%以上。结论我院鲍曼不动杆菌具有同源相关性,应用多位点序列分型技术对多重耐药的鲍曼不动杆菌实时监测,严加预防。Objective To investigate the molecular epidemiology characteristic and analyze gene homology of Acinetobacter baumannii isolated in each ward of our hospital, so to provide the knowledge evidences for nosocomial infectioncontrol, and the application of themultilocus sequence typing(MLST) technology.Methods 50 A. baumanni stains were collected from our hospital, and the genotype was identified by multilocus sequence typing(MLST) technique. gltA, gyrB, gdhB, recA, cpn60, gpi and rpoD housekeeping genes were detected by PCR(Polymerase Chain Reaction) amplification. PCR results were then sequenced andcompared with PubMLST databases. Evolutionary diagram was generated with Phyloviz 2. 0 software.Results Tengenotypes were identified among 50 A. baumannii stains: ST-1696(19 strains),ST-1144(14 strains), ST-191(4 strains), ST-229( 4 strains)and ST-540, ST-1680,208, ST-1679, ST-1145, ST-1421(1 strains), respectively, and three new genes(3 strains). Among them, ST-1696, and ST-1144, ST-191,ST-540, ST-1680, ST-208, ST-1679, ST-1145, ST-1421 genotype have the homologous relevance. Drug sensitivity test results showed that most of 47 strains of acinetobacterbaumannii strains had multiple drug resistance of clinical commonly used antibiotics, and the antibiotic resistant rate were over 75%. Conclusion Acinetobacter baumannii isolated in our hospital have the homologous correlation, and MLSTcould be used in genotyping of clinical Acinetobacter baumannii. Multiple drug resistance of acinetobacterbaumanniishould be real-time detected and prevented.

关 键 词：鲍曼不动杆菌 医院感染 同源性 多位点系列分型技术 

分 类 号：R446.5[医药卫生—诊断学]