miRNA-146a在肾移植后感染HPV-B19患者中的表达  

The Expression of miRNA-146a in HPV-B19 Infected Patients After Renal Transplantation

作　　者：唐倩[1] 朱蕾[1] 邓先平[1] 刘欣欣[1] TANG Qian;ZHU Lei;DENG Xian-ping;LIU Xin-xin(Department of Clinical Laboratory,The 309th Hospital of PLA,Beijing 100091,China)

机构地区：[1]解放军第309医院检验科,北京100091

出　　处：《标记免疫分析与临床》2018年第11期1619-1622,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨肾移植术后患者与HPV-B19的关系及miRNA-146a在移植后感染HBV-B19患者中的意义。方法选取34例接受肾移植的患者,分别在移植后3、7、10、15、25天留取血清标本作为移植组,留取移植前血清作为对移植前对照组,20例健康人员正常对照组,用ELISA方法检测HBV-B19 IgM,3组间应用统计学方法进行分析;移植后感染组与非感染组比较,用PCR方法检测miRNA-146a的表达差异及相关性分析。结果移植组(11.16±4.41)分别与移植前对照组(8.39±2.09)和正常对照组(7.74±2.96)比较,差异具有统计学意义(P<0.05);移植前对照组(8.39±2.09)与正常对照组(7.74±2.96)比较,差异无统计学意义(P>0. 05);移植后感染组RE值(2.48×102±265.49)明显高于非感染组(4.77±2.23),P值为0.013,差异具有统计学意义。microRNA-146a的表达量与HPV-B19具有相关性。结论患者肾移植后,感染HBV-B19的机率明显增加,对预后有一定影响,miRNA-146a可以作移植后感染HBV-B19的重要指标,在监测移植后的发生发展有着重要意义。Objective To investigate the states of human p arovirus (HPV)-B19 infection of recipients after the renal transplantation,and to study the functional mechanism of miRNA 146 in HPV-B19 infection of recipients after the renal transplantation.Methods We obtained patient serum samples at 3,7,10,15 and 25 days after the transplantation in 34 renal transplant recipients as the study group,while 34 samples before the transplantation were used as the control group for non-renal transplant and also 20 cases of healthy controls group.The HPV-B19 IgM ELISA assay was conducted in blood serum and we thencompared the results among three groups;PCR was used to detect the levels of microRNA-146a for further comparison.Results The relative expression of transplantation recipients group (11.16±4.41) was significantly higher than pre transplantation control group (8.39±2.09) and then healthy control group (7.74±2.96),P <0.05;The relative expression of miRNA-146a in active HPV-B19 infection group (Relative Expression,RE)(2.48×10^2±265.49) was significantly higher than that of the taintless HPV-B19 group (4.77±2.23),P =0.013. The correlation coefficient between the expression of miRNA-146a and HPV-B19 infection group was r=0.076,P <0.05.Conclusion There is a higher chance of HPV-B19 infection after the renal transplantation. miRNA-146a can be used as a potential diagnostic marker in HPV-B19 infection of recipients after the renal transplantation.

关 键 词：HPV-B19 肾移植 miRNA-146a 

分 类 号：R699.2[医药卫生—泌尿科学] R446.6[医药卫生—外科学]