大熊猫源杯梗孢属真菌(Cyphellophora pluriseptata)的分离鉴定与致病性研究  

Isolation, identification and pathogenicity of Cyphellophora pluriseptata from Giant panda

作　　者：马晓平[1] 姜尧章 张和民 古玉[3] 曹三杰[1] 王承东 左之才[1] 李德生 凌珊珊 夏介英[4] 王平峰 MA Xiao-ping;JIANG Yao-zhang;ZHANG He-min;GU Yu;CAO San-jie;WANG Cheng-dong;ZUO Zhi-cai;LI De-sheng;LING Shan-shan;XIA Jie-ying;WANG Ping-feng(College of Veterinary Medicine of Sichuan Agricultural University,Chengdu 611130,China;China Conservation and Research Center for Giant Panda,Ya'an 625000,China;College of Life Science,Sichuan Agricultural University,Ya'an 625014,China;Sichuan academy of Chinese Medicine,Chengdu 610041,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]中国大熊猫保护研究中心,四川雅安625000 [3]四川农业大学生命科学院,四川雅安625014 [4]四川省中医药科学院,四川成都610041

出　　处：《中国预防兽医学报》2018年第11期1066-1070,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：大熊猫国际资金资助项目(AD1415);四川省科技厅应用基础研究计划项目(2018JY0183)

摘　　要：为探究大熊猫真菌性皮肤病的病因,取发生肉芽肿大熊猫患部的皮屑、被毛进行真菌学检查和病原分离。采用沙氏培养法对采集的皮屑和毛发样品进行分离纯化,对分离株进行形态学、分子生物学鉴定和小鼠致病性试验及药敏试验。结果显示,从样品中分离到一株真菌(JYZ030101)。对分离株JYZ030101的ITS区进行PCR扩增获得长度为617 bp片段,经Blast比对结果显示该序列与杯梗孢属真菌Cyphellophora pluriseptata的相似性为99%。结合形态学和分子生物学方法将分离菌鉴定为C.pluriseptata。动物致病性试验显示该菌对小鼠有选择性致病作用。药敏试验显示该菌对特比萘芬、两性霉素B、伊曲康唑和氟胞嘧啶耐药,对氟康唑、伏立康唑敏感。本研究为诊断和治疗C.pluriseptata引起的大熊猫真菌性皮肤病提供重要参考。In order to investigate the causes of fungal dermatosis in giant pandas,the scarf of skin granuloma in the affected giant panda was taken to the fungal examination,and a fungi was isolated from the scarf,designated JYZ030101,which was subjected to the morphological characteristics and internal transcribed spacer (ITS)sequence identification,pathogenicity test in mice,and drug sensitive examination.The result showed that the length of the ITS sequence was 617bp,which was submitted to GenBank and obtained the accession number of KX283137.NCBI blast result showed that JYZ030101had 99%similarity with Cyphellophora pluriseptata.According to the morphological identification,ITS sequence and phylogenetic tree analysis,the isolate was identified as C.pluriseptata.The pathogenicity test revealed that the isolate had a selective pathogenesis to mice skin.The drug susceptibility test showed that the C.pluriseptata was resistant to terbinafine,amphotericin B,itraconazole and fiuorouracil,but sensitive to fluconazole and voriconazole.This study provides an important reference for the diagnosis and treatment of C.pluriseptata caused disease.

关 键 词：杯梗孢属 形态学 ITS序列 致病性 药敏试验 

分 类 号：S852.61[农业科学—基础兽医学]