大黄素对高脂饮食诱导的肥胖小鼠肾脏损害的保护作用  

Emodin alleviated kidney damage in high fat diet induced obese mice

作　　者：孙耀 于亚丽 李江 张海鸥 Sun Yao;Yu Yali;Li Jiang;Zhang Haiou(Department of Pharmacy,Taikang Xianlin Drum Tower Hospital,Nanjing 210000,China)

机构地区：[1]泰康仙林鼓楼医院药剂科,南京210000 [2]锦州医科大学附属第一医院内分泌科 [3]浙江省中医院药剂科

出　　处：《中华内分泌代谢杂志》2018年第11期946-950,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：辽宁省自然科学基金(201602308).

摘　　要：目的 研究大黄素(emodin,EM)对高脂饮食诱导的肥胖小鼠肾脏损伤的影响.方法 雄性C57bl/6小鼠根据饮食和给药不同分组.Western印迹法检测各组小鼠肾脏Ⅳ型胶原(collagenⅣ,Col4),磷酸肌醇3-激酶(phosphatidylinositide 3-kinase,PI3K),磷酸化的磷酸肌醇3-激酶(phosphorylated phosphoinositol 3-kinase,p-PI3K),蛋白激酶B(protein kinase,Akt),磷酸化的蛋白激酶B(phosphorylated protein kinase,p-Akt).结果 肥胖小鼠尿蛋白排泄增加,Col4在肾脏沉积增加,p-PI3K和p-Akt在肾脏表达明显减少.大黄素治疗的肥胖小鼠尿蛋白排泄减少,Col4在肾脏沉积减少,p-PI3K和p-Akt在肾脏表达明显增加.结论 大黄素可能通过改善肾脏局部的胰岛素抵抗减轻高脂饮食诱导的肥胖小鼠肾脏损伤.Objective To explore the protective effect of emodin on kidney damage in high-fat diet-induced obese mice. Methods Male C57bl/ 6 mice were divided into groups according to diet and treatment. Collagen Ⅳ(Col4), phosphatidylinositide 3-kinase(PI3K), phosphorylated phosphoinositol 3-kinase(p-PI3K), protein kinase (Akt), and phosphorylated protein kinase(p-Akt) were measured by Western blotting method. Results Col4 was increased, while p-PI3K and p-Akt were decreased in kidney tissue in high-fat diet-induced obese mice. However, there was lower level of Col4, but higher levels of p-PI3K and p-Akt in kidneys. Conclusion Kidney damage of high-fat diet-induced obese mice seems to be alleviated by emodin via improving insulin sensitivity.

关 键 词：肥胖症 大黄素 肾脏损伤 胰岛素抵抗 Ⅳ型胶原 

分 类 号：R285.5[医药卫生—中药学]