镍钛三维记忆合金网复合自体骨治疗比格犬胫骨平台塌陷活体模型的生物力学试验  

Biomechanical study on nickel-titanium three-dimensional memory alloy mesh combined with autologous bone for living model of canine tibial plateau collapse fracture

作　　者：严新安 姚相雨 方跃[1] 梁羽[1] 杨云 黄富国[1] YAN Xin'an;YAO Xiangyu;FANG Yue;LIANG Yu;YANG Yun;HUANG Fuguo(Department of Orthopedics,West China Hospital,Sichuan University,Chengdu Sichuan,610041,P.R.China)

机构地区：[1]四川大学华西医院骨科,成都610041

出　　处：《中国修复重建外科杂志》2018年第12期1549-1553,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：四川省科技厅支撑项目(2012FZ0010)~~

摘　　要：目的通过生物力学试验探讨镍钛三维记忆合金网复合自体骨治疗比格犬胫骨平台塌陷型骨折活体模型的效果。方法将16只健康12月龄比格犬随机分为4组,每组4只。A、B、C组建立胫骨平台塌陷型骨折模型。造模后4 h内,A、B、C组分别于骨缺损区植入三维记忆合金网加自体骨(腓骨皮质骨粒)、人工合成骨(纳米羟基磷灰石颗粒)或自体骨,然后在骨缺损区外侧行钢板螺钉固定。D组犬不作任何处理,作为正常对照。术后5个月处死所有动物,取标本行大体观察后,采用生物力学试验机行破坏性轴向压缩试验,记录标本断裂时位移及最大失效载荷,计算轴向刚度(即载荷/位移)。结果术后各组动物均无死亡,切口愈合良好;术后1～3 d即可自行站立活动,未见明显患肢畸形等异常。术后5个月,大体观察见各组胫骨平台标本关节面均完整光滑,未见关节面明显塌陷。A、D组标本最大失效载荷及轴向刚度均显著高于B、C组,差异有统计学意义(P<0.05);A、D组间及B、C组间比较差异均无统计学意义(P>0.05)。结论镍钛三维记忆合金网复合自体骨修复犬胫骨平台关节面下骨缺损,术后5个月时可获得良好的生物力学性能,与人工合成骨和自体骨移植相比有更好的轴向刚度。Objective To evaluate the effect of nickel-titanium three-dimensional memory alloy mesh combined with autologous bone for living model of canine tibial plateau collapse fracture by biomechanical testing. Methods Sixteen healthy 12-month-old Beagle dogs were randomly divided into 4 group, 4 dogs in each group. The dogs were used to establish the tibial plateau collapse fracture model in groups A, B, and C. Then, the nickel-titanium three-dimensional memory alloy mesh combined with autologous bone(the fibula cortical bone particles), the artificial bone(nanohydroxyapatite),and autologous fibula cortical bone particles were implanted to repair the bone defects within 4 hours after modeling in groups A, B, and C, respectively; and the plate and screws were fixed outside the bone defects. The dogs were not treated in group D, as normal control. At 5 months after operation, all animals were sacrificed and the tibial specimens were harvested and observed visually. The destructive axial compression experiments were carried out by the biomechanical testing machine. The displacement and the maximum failure load were recorded and the axial stiffness was calculated. Results All animals stayed alive after operation, and all incisions healed. After 1-3 days of operation, the animals could stand and move, and no obvious limb deformity was found. The articular surfaces of the tibial plateau specimens were completely smooth at 5 months after operation. No obvious articular surface collapse was observed. The displacement and maximum failure load of specimens in groups A and D were significantly higher than those in groups B and C(P<0.05). But no significant difference was found between groups A and D and between groups B and C(P>0.05). Conclusion The nickel-titanium three-dimensional memory alloy mesh combined with autologous bone for subarticular bone defect of tibial plateau in dogs has good biomechanical properties at 5 months after operation, and has better axial stiffness when compared with the artificial bone and autologous

关 键 词：镍钛记忆合金 胫骨平台骨折 比格犬 生物力学 

分 类 号：R687[医药卫生—骨科学] R-332[医药卫生—外科学]