HPLC法测定玉叶解毒颗粒中绿原酸和槲皮素两种主要成分的含量  

Determination of Content of Chlorogenic Acid and Quercitrin in Yuye Granules by HPLC

作　　者：杨立芳 郭洋洋 黄美玲 陈美余 姜明国 石松标 王芳 吴家法 吴叶宇 谭学才 YANG Li-fang;GUO Yang-yang;HUANG Mei-ling;CHEN Mei-yu;JIANG Ming-guo;SHI Song-biao;WANG Fang;WU Jia-fa;WU Ye-yu;TAN Xue-cai(School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Nanning 530006,China;School of Marine Sciences and Biotechnology,Guangxi University for Nationalities,Nanning 530006,China)

机构地区：[1]广西民族大学化学化工学院,广西南宁530006 [2]广西民族大学海洋与生物技术学院,广西南宁530006

出　　处：《时珍国医国药》2018年第10期2346-2348,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81560713);广西自然科学基金(2016GXNSFAA380075)

摘　　要：目的建立同时测定玉叶解毒颗粒中绿原酸和槲皮素两种主要成分含量的HPLC方法。方法采用Phenomenonex Gemini C18色谱柱(250 mm×4. 6 mm,5μm),以甲醇(A)-0. 4%磷酸水溶液(B)为流动相,梯度洗脱(0～20 min,80%A→55%A; 30～45 min,15%A→0%A),流速1 m L/min,检测波长为345 nm。结果玉叶解毒颗粒中绿原酸、槲皮素的含量范围分别为:0. 30～3. 00μg/g,0. 18～1. 8μg/g,且线性关系良好;平均回收率分别为99. 930%、95. 005%,RSD均小于2. 0%。结论该检测方法简便,可靠,稳定。经方法学验证,可用于同时检测玉叶解毒颗粒中绿原酸和槲皮素的含量。Objective To establish an HPLC method for simutaneous determination of chlorogenic and quercitrin in Yuye Gran- ules.Methods HPLC experiments were performed on a Phenonmenonex Gemini C18 column (250mm×4.6mm,5μm),the mobile phase consisted of methanol (A)-0.4%phosphoric acid (B)in gradient mode (0-20min,80%A -55% A;30-45 min,15%A-0%A),the flow rate was 1mL/min.The detection wavelength was set at 345nm.Results The contents of chlorogenic acid and quericitrin in Yuye Granules were 0.30-3.00μg/g and 0.18-1.8μg/g,showed a good linear relation- ship.The average recoveries of chlorogenic and quercitrin were 99.930%and 95.005%with RSD below 2.0%. Conclusion The detection method was simple,reliable,stable.The establish method can be used in dertermination of chlorogenic acid and quercitrin in Yuye Granules.

关 键 词：玉叶解毒颗粒 绿原酸 槲皮素 HPLC 

分 类 号：R284.1[医药卫生—中药学]