对叶百部遗传多样性的AFLP分析  

AFLP analysis on genetic diversity of traditional medicinal plant Stemona tuberosa

作　　者：杨丹丹[1] 王晓彤[1] 吴思宇[1] 王孝勋[1] 张莹 傅静 YANG Dan-dan;WANG Xiao-tong;WE Si-yu;WANG Xiao-xun;ZHANG Ying;FU Jing(Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《时珍国医国药》2018年第10期2519-2521,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81360640);广西自然科学基金项目(2016GXNSFAA380072);广西壮瑶药重点实验室(桂科基字[2014]32号);壮瑶药协同创新中心(桂教科研[2013]20号);广西重点学科(壮药学)资助项目(桂教科研[2013]16号);广西八桂学者"中药创新理论与药效研究"(桂科政字[2013]25号)

摘　　要：目的对对叶百部Stemona tuberosa Lour.不同居群进行遗传多样性和遗传结构进行分析。方法利用荧光AFLP标记技术,8对EcoR I/Mse I引物组合对38份供试材料的基因DNA扩增,进行多态性检测,用NTSYSpc-2. 11F软件进行数据分析。以DICE相似系数为基础而得出UPGMA个体间聚类图和相似系数。结果共获得条带数1477条,多态性条带数1477条,多态性达到了100%。UPGMA个体间聚类结果表明,对叶百部种群间的遗传距离与地理距离有相关性。结论对叶百部不同居群间的遗传多样性非常丰富。为对叶百部种质资源的合理利用和品种选育提供了分子水平的理论依据。Objective To analyze the genetic diversity and genetic structure of different population of Stemona tuberosa Lour.Methods 8 primer combinations(EcoR I/Mse I) were used to analyze the Amplified Fragment Length Polymorphism(AFLP) in38 test materials. Polymorphisms were detected and the data was analyzed by NTSYSpc-2. 11 F software,then UPGMA clustering figure and similarity coefficient based on DICE were given. Results A total of 1477 polymorphic bands were dectected and the polymorphism was 100%. The clustering results showed that there was a correlation between the genetic distance and geographical distance of Stemona tuberosa Lour. populations. Conclusion There were high genetic diversity and rich polymorphism in Stemona tuberosa Lour. population. The study provided a theoretical basis at the molecular level for the rational use of germplasm resources of Stemona tuberosa Lour. and their new variety breeding.

关 键 词：对叶百部 AFLP 遗传多样性 

分 类 号：R284.1[医药卫生—中药学]