广西地不容中生物碱类成分的含量测定  

Determination of Alkaloids in Diburong(Stephania Epigaea Lo) by HPLC

作　　者：黄秋洁 王捷 叶勇[4] 王志萍 韦炜 HUANG Qiu-jie;WANG Jie;YE Yong;WANG Zhi-ping;WEI Wei(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning Guangxi 530001,China;Key Labaratory of Research and Development Gemeric Teehology,TCM Preparation in Guangxi Universities,Nanning Guangxi 530001,China;Pharmaceutical Factory of Guangxi Medical University,Nanning Guangxi 530021, China;School of Pharmacy,Guangxi Medical University,lVanning Guangxi 530021,China)

机构地区：[1]广西中医药大学药学院,广西南宁530001 [2]广西高校中药制剂共性技术研发重点实验室,广西南宁530001 [3]广西医科大学制药厂,广西南宁530021 [4]广西医科大学药学院,广西南宁530021

出　　处：《中医药导报》2018年第19期81-84,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西高校科技创新能力提升工程建设项目(70-ZJGX201402003);广西教育厅科研基金(LX2014062);广西大学生创新创业训练计划项目(201510598063)

摘　　要：目的:建立广西地不容中延胡索乙素、巴马汀和青藤碱含量测定方法。方法:以HypersilODS2柱(4.6 mm×250 mm,5μm)为色谱柱,流动相:乙腈-0.5%磷酸水溶液,梯度洗脱,检测波长:282 nm,流速:1.0 mL·min-1,柱温:30℃,进样量:10μL。结果:延胡索乙素、巴马汀、青藤碱分别在0.135～1.35 mg·mL-1、0.032,98～0.329,8 mg·mL-1、0.031,24～0.312,4 mg·mL-1范围内线性关系良好,r=0.999,9。结论:该方法准确、可靠,可用于广西地不容药材及相关制剂中生物碱类成分的含量测定。Objective:To establish an HPLC method for determination of tetrahydropalmatine,palmatine and sinomenine in Diburong (Stephania epigaea Lo).Methods:Using HypersilODS2(4.6mm×250mm,5μm)as ana- lytical column.The mobile phase consisted of acetonitrile-0.5% phosphoric acid with gradient elution.The de- tection wavelength was 282nm,The flow rate was 1.0mL·min^-1;Column temperature was at 30℃.Results:The linear calibration curve of tetrahydropalmatine,palmatine and sinomenine were obtained in the concentration range of 0.135-1.35mg·mL^-1,0.032,98-0.329,8mg·mL^-1 and 0.031,24-0.312,4 mg·mL^-1(r=0.999,9).Conclusion: The method is accurate and reliable.It is appropriate for the quantitative determination of alkaloids in (Stepha- nia epigaea Lo)and it's preparation.

关 键 词：广西地不容 延胡索乙素 巴马汀 青藤碱 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]