HPCE测定附子活性成分的血浆蛋白结合率  

Study on the Plasma Protein Combine Rate of the Active Compounds of Aconiti Lateralis Radix Praeparata by HPCE

作　　者：梁勇[1] 郭鹏[2] 陈作[1] 李艳灵[1] 郝征[3] 高克俭[1] LIANG Yong;GUO Peng;CHEN Zuo;LI Yanling;HAO Zheng;GAO Kejian(Tianjin Beichen District Hospital of Traditional Chinese Medicine,Tianjin 300400,China;Tianjin Children's Hospital,Tianjin 300134,China;Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津市北辰区中医医院,天津300400 [2]天津市儿童医院,天津300134 [3]天津中医药大学,天津300193

出　　处：《中国现代应用药学》2018年第11期1632-1636,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：天津市北辰科技发展计划重点项目(2016-SHGY-19;2015-SHGY-20)

摘　　要：目的建立适用于附子生物碱类化学成分血浆蛋白结合率的方法。方法通过考察进样方式、电泳缓冲液组成等因素确定色谱条件,并采用该条件测定苯甲酰次乌头原碱、苯甲酰新乌头原碱和苯甲酰乌头原碱的血浆蛋白结合率。结果经考察确定色谱条件为:电压进样,缓冲液为100 mmol·L^(-1)硼砂-硼酸缓冲溶液(pH=9.0)∶甲醇=7∶3,分离电压为15 kV,检测波长为230 nm。在1,2,5μg·mL^(-1)3个剂量下,附子中苯甲酰次乌头原碱的蛋白结合率分别为(43.81±3.2)%,(42.51±3.6)%,(42.09±2.3)%;苯甲酰新乌头原碱的蛋白结合率分别为(39.59±4.0)%,(34.58±3.7)%,(37.02±3.0)%;苯甲酰乌头原碱的蛋白结合率分别为(48.23±4.6)%,(33.26±3.9)%,(32.60±3.8)%。结论超滤法结合HPCE技术可作为一种新型药物蛋白结合率测定的方法。OBJECTIVE To establish a method for evaluating the plasma protein binding rate of alkaloid in Aconiti Lateralis Radix Praeparata. METHODS The chromatography condition such as sample injection condition and the ingredient of electrophoretic buffer solution were evaluated. And the plasma protein binding rate of benzoylhypaconine, benzoylaconine and benzoylmesaconine were analyzed. RESULTS The chromatography condition was as followes: voltage injection, electrophoretic buffer solution [100 mmol·L-1 borate saline buffer(pH=9.0)∶menthol=7∶3], separation voltage was 15 kV, the detection wavelength was 230 nm. Within the level of 1, 2, 5 μg·mL-1, the plasma protein binding rates of benzoylhypaconine were(43.81±3.2)%,(42.51±3.6)%,(42.09±2.3)%; the plasma protein binding rates of benzoylaconine were(39.59±4.0)%,(34.58±3.7)%,(37.02±3.0)%; and the plasma protein binding rates of benzoylmesaconine were(48.23±4.6)%,(33.26±3.9)%,(32.60±3.8)%. CONCLUSION Ultrafiltration combined with HPCE technology can be used as a new method for the analysis of plasma protein binding rate.

关 键 词：高效毛细管电泳 超滤 附子 血浆蛋白 

分 类 号：R917[医药卫生—药物分析学]