骨髓增生异常综合征患者骨髓单个核细胞WT1基因表达的研究  

Expression of WT1 gene in bone marrow mononuclear cells of patients with myelodysplastic syndromes

作　　者：谢静[1] 黄玉娟 石培民[1] Xie Jing;Huang Yujuan;Shi Peimin(Department of Hematology,Taixing People's Hospital,Taixing 225400,China)

机构地区：[1]江苏省泰兴市人民医院血液科,225400

出　　处：《白血病．淋巴瘤》2018年第11期675-677,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 分析骨髓增生异常综合征(MDS)患者骨髓单个核细胞中WT1基因的表达与细胞遗传学特点、疗效及预后的关系.方法 采用实时荧光定量聚合酶链反应(RQ-PCR)检测2010年8月至2018年3月江苏省泰兴市人民医院收治的115例初诊MDS患者骨髓单个核细胞中WT1基因表达水平,分析其与细胞遗传学特点、治疗效果及预后的关系.结果 WT1基因高表达80例(69.6 %),低表达15例(13.0 %),未表达20例(17.4 %). 80例WT1高表达患者中,难治性血细胞减少伴原始细胞增多(RAEB)56例,难治性血细胞减少伴多系病态造血(RCMD)22例,难治性血细胞减少伴单系病态造血(RCUD)和MDS不能分类(MDS-U)各1例. 55例细胞遗传学预后非常差患者中,WT1基因高表达44例;28例预后差患者中WT1基因高表达19例. WT1基因高表达组完全缓解率为12.5 %(10/80),低于低表达组的26.7 %(4/15)及未表达组的40.0 %(8/20),三组完全缓解率差异有统计学意义(χ^2=8.96,P<0.05).结论 MDS患者中WT1基因高表达者缓解率低,预后差.Objective To analyze the relationship between the expression of WT1 gene in bone marrow mononuclear cells and the cytogenetic characteristics, curative effect and prognosis in patients with myelodysplastic syndromes (MDS). Methods The quantitative expression of WT1 gene was detected by real-time quantitative polymerase chain reaction (RQ-PCR) in bone marrow mononuclear cells of 115 MDS patients who were admitted to Taixing People's Hospital from August 2010 to March 2018, and the relationship between the expression of WT1 gene and the cytogenetic characteristics, curative effect and prognosis were evaluated. Results WT1 gene was highly expressed in 80 cases (69.6 %), lowly expressed in 15 cases (13.0 %), and unexpressed in 20 cases (17.4 %). Of the 80 patients with high WT1 expression, 56 cases (70.0 %) were refractory anemia with excess blasts (RAEB), and 22 cases (27.7 %) were refractory cytopenia with multilineage dysplasia (RCMD). In 55 patients with very poor cytogenetic prognosis, 44 patients had high expression of WT1 gene, and in 28 patients with poor prognosis, 19 patients had high expression of WT1 gene. The complete remission rate of WT1 gene high expression group [12.5 % (10/80)] was lower than that of low expression group [26.7 % (4/15)] and unexpressed group [40.0 % (8/20)], and the difference of complete remission rate between the three groups was statistically significant (χ^2= 8.96, P< 0.05). Conclusion MDS patients with high expression of WT1 gene have low remission rate and poor prognosis.

关 键 词：骨髓增生异常综合征 WT1基因 细胞遗传学 预后 治疗结果 

分 类 号：R551.3[医药卫生—血液循环系统疾病]